Jabrane-Ferrat Nabila, El Costa Hicham
Toulouse Institute for Infectious and Inflammatory Diseases (Infinity), INSERM-CNRS-University Toulouse III, Toulouse, France.
Methods Mol Biol. 2022;2407:205-213. doi: 10.1007/978-1-0716-1871-4_15.
The human decidua basalis, main uterine mucosa during pregnancy, provides an ex vivo model for studying natural protection of macrophages against HIV-1 infection at the mucosal level. Beyond pregnancy, the decidua constitutes also a valuable tool to assess tissue-resident macrophage infection. Here, we provide a detailed protocol for decidual macrophage purification and tissue infection.
人胎盘基底层，孕期主要的子宫黏膜，为研究巨噬细胞在黏膜水平对HIV-1感染的天然保护作用提供了一个体外模型。除孕期外，胎盘基底层也是评估组织驻留巨噬细胞感染的一个有价值的工具。在此，我们提供了一份详细的胎盘巨噬细胞纯化及组织感染实验方案。
