抗乙醇诱导的胃溃疡的保护作用及其机制。
Protective effect of against ethanol-induced gastric ulcer and its mechanism.
机构信息
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
出版信息
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2021 Oct 25;50(5):561-567. doi: 10.3724/zdxbyxb-2021-0055.
: To investigate the protective effect of (FD) against ethanol-induced gastric ulcer and its mechanism. : Human gastric epithelial GES-1 cells were divided into normal control group, model control group, FD 95% alcohol extract group, FD 50% alcohol extract group and FD decoction extract group. Gastric ulcer was induced by treatment with 1% ethanol in GES-1 cells. The cell proliferation was detected with MTT method in each group. Sixty SD rats were randomly divided into normal control group, model control group, ranitidine group and low-dose, medium-dose, high-dose FD 95% alcohol extract groups (150, 300, 600 mg/kg). The corresponding drugs were administrated by gavage for The gastric ulcer model was induced by intragastric administration of anhydrous ethanol. The gastric ulcer area and ulcer inhibition rate of rats were measured in each group; the degree of gastricmucosal damage was observed by scanning electron microscopy; the levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β in serum and the content of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), catalase (CAT) in gastric tissues were detected by ELISA method. : 95% alcohol extract of FD had the strongest protective effect on proliferation of GES-1 cells. In animal experiments, compared with the normal control group, a large area of ulcers appeared on the gastric mucosa in the model control group, while the ulcer areas of the FD groups and ranitidine group were significantly smaller than that of the model control group (all <0.05). Compared with the model control group, FD groups and ranitidine group significantly reduced the levels of TNF-α, IL-1β, IL-6 in serum and the MDA content in the gastric tissues, and increased the activity of SOD, CAT and GSH in gastric tissues (all <0.05). : The 95% alcohol extract of FD can reduce the levels of TNF-α, IL-1β and IL-6 in serum and the content of MDA in gastric tissues, and increase the activity of SOD, CAT and GSH in gastric tissues to achieve the protective effect against gastric ulcer.
为了研究（FD）对乙醇诱导的胃溃疡的保护作用及其机制。：将人胃上皮 GES-1 细胞分为正常对照组、模型对照组、FD 95%醇提组、FD 50%醇提组和 FD 水煎煮组。用 1%乙醇处理 GES-1 细胞诱导胃溃疡。用 MTT 法检测各组细胞增殖。将 60 只 SD 大鼠随机分为正常对照组、模型对照组、雷尼替丁组和 FD 95%醇提低、中、高剂量组（150、300、600mg/kg）。灌胃给予相应药物，通过灌胃诱导无水乙醇致大鼠胃溃疡模型。测量各组大鼠的胃溃疡面积和溃疡抑制率；扫描电镜观察胃黏膜损伤程度；用 ELISA 法检测血清肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6、IL-1β水平及胃组织丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、过氧化氢酶（CAT）含量。：FD 95%醇提取物对 GES-1 细胞增殖的保护作用最强。动物实验中，与正常对照组相比，模型对照组胃黏膜出现大面积溃疡，而 FD 组和雷尼替丁组的溃疡面积明显小于模型对照组（均<0.05）。与模型对照组相比，FD 组和雷尼替丁组血清 TNF-α、IL-1β、IL-6 水平及胃组织 MDA 含量降低，胃组织 SOD、CAT、GSH 活性升高（均<0.05）。：FD 的 95%醇提取物可降低血清 TNF-α、IL-1β 和 IL-6 水平及胃组织 MDA 含量，提高胃组织 SOD、CAT 和 GSH 活性，从而达到抗胃溃疡的保护作用。
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