Lin Tao, Li Qianhui, Jin Defu, Liu Wanbo, Tang Chaogui, Zhang Xiaoyun
Department of Medical Laboratory, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huanghe West Road 1, Huai'an, Jiangsu 223300, China.
Can J Infect Dis Med Microbiol. 2021 Dec 26;2021:5354747. doi: 10.1155/2021/5354747. eCollection 2021.
is the major pathogen causing nosocomial human infections and produces a variety of virulence factors that contribute to its ability to colonize and cause diseases. This study was conducted to investigate the virulence genes in isolated from sterile body fluid samples and their correlation with clinical symptoms and outcomes. The VITEK 2® Compact system was used to perform biochemical identification and antimicrobial susceptibility tests on 33 isolates. Virulence genes were amplified using multiplex PCR. The virulence gene patterns were analyzed by systematic cluster analysis. The frequency of methicillin-resistant was 45.45%, and 17 virulence genes were identified. Genes encoding hemolysins showed high frequencies. The frequencies of , , , and were 93.94% and that of the was 21.21%. Except for the frequency of (51.52%), the remaining genes encoding invasive proteases showed frequencies greater than 81.82%. Among the patients, 100.00% had undergone invasive medical procedures and 24.00% had been treated with more than three types of antibiotic drugs. Invasive medical procedures are the main causes of infection. Resistance to antibiotic drugs and the status of carrying virulence genes were highly related to clinical symptoms and outcomes.
是引起医院内人类感染的主要病原体，可产生多种毒力因子，有助于其定植和致病。本研究旨在调查从无菌体液样本中分离出的的毒力基因及其与临床症状和结局的相关性。使用VITEK 2® Compact系统对33株分离株进行生化鉴定和药敏试验。采用多重PCR扩增毒力基因。通过系统聚类分析毒力基因模式。耐甲氧西林的频率为45.45%，鉴定出17个毒力基因。编码溶血素的基因频率较高。、、和的频率为93.94%，的频率为21.21%。除（51.52%）外，其余编码侵袭性蛋白酶的基因频率均大于81.82%。患者中，100.00%接受过侵入性医疗操作，24.00%接受过三种以上抗生素治疗。侵入性医疗操作是感染的主要原因。对抗生素的耐药性和携带毒力基因的状况与临床症状和结局高度相关。
