The International Center for Advanced Researches (ICTAR), Giza, Egypt.
Department of Chemistry, Faculty of Science, Cairo University, Giza, Egypt.
J Gastrointest Cancer. 2022 Dec;53(4):888-901. doi: 10.1007/s12029-021-00762-3. Epub 2022 Jan 6.
The present study aimed to evaluate the anticancer potential of Egyptian scorpion Leiurus quinquestriatus venom (ScV) or human Wharton's jelly-derived mesenchymal stem cells conditioning medium (hWJ-MSCs-CM)/CM against breast cancer (MCF-7) cell line as an alternative effective cancer biotherapy.
Venom (ScV) toxicity was performed recording concentration-dependent viability % and ScV IC value was in the order of 100 μg/ml. MCF-7 were treated with hWJ-MSCs-CM used as (25%, 50%, and 75% ml) or the IC of ScV. Apoptotic activity was traced via evaluation the apoptotic (Bax, Casp-3, and Casp-9) and anti-apoptotic genes (Bcl2, ALDOA, and PKM2) profile.
Both Bax and Casp-3 showed a significant upregulation while anti-apoptotic genes were significantly downregulated. In the meantime, Casp-3 and Casp-9 protein were monitored using ELISA, and their level was less than in control. Additionally, MCF-7 apoptosis was monitored using flow cytometry recording a significant DNA accumulation in the G0-G1 and S phases in case of cell treatment with ScV or CM75% ml and 50% ml. Also, there was a significant total necrotic cells % compared with control cells, and total apoptosis under the effect of ScV or CM75% ml was significantly elevated than rest of treatment.
Apoptosis induction was both dose- and time-dependent for hWJ-MSCs-CM and ScV. According to the present study and other studies, there is an ample evidence that hWJ-MSCs-CM and the venom IC abolish tumor growth.
本研究旨在评估埃及蝎子 Leiurus quinquestriatus 毒液（ScV）或人脐带来源间充质干细胞条件培养基（hWJ-MSCs-CM）/CM 对乳腺癌（MCF-7）细胞系的抗癌潜力，作为一种替代有效的癌症生物疗法。
记录浓度依赖性细胞活力%，评估毒液（ScV）毒性，ScV 的 IC 值为 100μg/ml。用 hWJ-MSCs-CM（25%、50%和 75% ml）或 ScV 的 IC 处理 MCF-7。通过评估凋亡（Bax、Casp-3 和 Casp-9）和抗凋亡基因（Bcl2、ALDOA 和 PKM2）谱，追踪细胞凋亡活性。
Bax 和 Casp-3 均显著上调，而抗凋亡基因显著下调。同时，用 ELISA 监测 Casp-3 和 Casp-9 蛋白，其水平低于对照组。此外，用流式细胞术监测 MCF-7 细胞凋亡，记录到 ScV 或 CM75% ml 和 50% ml 处理细胞后 G0-G1 和 S 期的 DNA 显著积累。与对照细胞相比，总坏死细胞%也显著升高，ScV 或 CM75% ml 的总凋亡明显高于其他处理。
hWJ-MSCs-CM 和 ScV 的诱导凋亡均具有剂量和时间依赖性。根据本研究和其他研究，有充分的证据表明 hWJ-MSCs-CM 和毒液 IC 可抑制肿瘤生长。
