血清 miR-34a-5p 和 miR-199a-3p 作为新生儿败血症的新型生物标志物。
Serum miR-34a-5p and miR-199a-3p as new biomarkers of neonatal sepsis.
机构信息
Faculty of Medicine, Departments of Medical Biochemistry and Molecular Biology, Fayoum University, Fayoum, Egypt.
Faculty of Medicine, Departments of Pediatrics, Fayoum University, Fayoum, Egypt.
出版信息
PLoS One. 2022 Jan 6;17(1):e0262339. doi: 10.1371/journal.pone.0262339. eCollection 2022.
BACKGROUND
Neonatal sepsis is a serious condition. Recent clinical studies have indicated that microRNAs (miRNAs) are key players in the pathogenesis of sepsis, which could be used as biomarkers for this condition.
PATIENTS AND METHODS
A total of 90 neonates with sepsis and 90 healthy neonates were enrolled in this study. qRT-PCR was performed to measure the expression levels of serum miR-34a-5p and miR-199a-3p.
RESULTS
miR-34a-5p and miR-199a-3p serum levels were significantly reduced in neonates with sepsis compared with those in healthy neonates (P = 0.006 and P = 0.001, respectively). Significant correlations of miR-34a-5p and miR-199a-3p with each of TLC, RDW, RBS, and C-reactive protein (CRP) as well as SNAPII were observed, indicating their associations with the severity of neonatal sepsis.
CONCLUSION
miR-34a-5p and miR-199a-3p may be useful as novel biomarkers in neonatal sepsis and may provide a new direction for its treatment.
背景
新生儿败血症是一种严重的疾病。最近的临床研究表明，微小 RNA（miRNAs）是败血症发病机制中的关键因素，可作为该病的生物标志物。
患者和方法
本研究共纳入 90 例败血症新生儿和 90 例健康新生儿。采用 qRT-PCR 检测血清 miR-34a-5p 和 miR-199a-3p 的表达水平。
结果
败血症新生儿血清 miR-34a-5p 和 miR-199a-3p 水平明显低于健康新生儿（P = 0.006 和 P = 0.001）。miR-34a-5p 和 miR-199a-3p 与 TLC、RDW、RBS 和 C-反应蛋白（CRP）以及 SNAPII 均呈显著相关，表明其与新生儿败血症的严重程度有关。
结论
miR-34a-5p 和 miR-199a-3p 可作为新生儿败血症的新型生物标志物，为其治疗提供新方向。
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