Department of Pediatrics, The First People's Hospital of Yuhang, Zhejiang, Hangzhou, China.
Bioengineered. 2021 Dec;12(1):4174-4182. doi: 10.1080/21655979.2021.1955580.
Sepsis is a systemic inflammatory response caused by infection and is a major cause of neonatal death. This study explored the miR-455-5p in neonatal sepsis, and further investigated the diagnostic and prognostic value of miR-455-5p in neonatal sepsis (NS). The levels of serum miR-455-5p in 88 healthy controls and 90 NS patients were examined by quantitative real-time polymerase chain reaction (qRT-PCR). Pearson correlation coefficient was used to evaluate the correlation between miR-455-5p and clinical features. Receiver operating characteristic (ROC) curve analysis was performed for the diagnostic evaluation on miR-455-5p. The prognostic value of miR-455-5p in NS was analyzed by Kaplan-Meier survival curve and multivariate Cox regression. The expression of serum miR-455-5p in NS patients was highly expressed in comparison to healthy controls ( < 0.001), and the level of miR-455-5p was positively correlated with white blood cell count (WBC) and other clinical characteristics ( < 0.01). The AUC value of ROC curve was 0.895, suggesting that miR-455-5p had diagnostic value for NS. Survival analysis illustrated that patient with high miR-455-5p expression had poor prognosis (log rank = 0.015), and miR-455-5p may be a potential prognostic marker for NS (HR = 3.454, 95% CI = 1.165-10.234, = 0.025). The expression of miR-455-5p had the ability to distinguish NS from healthy people, and highly expressed miR-455-5p was associated with poor prognosis in NS patients.
败血症是由感染引起的全身炎症反应，是新生儿死亡的主要原因。本研究探讨了 miR-455-5p 在新生儿败血症中的作用，并进一步研究了 miR-455-5p 在新生儿败血症（NS）中的诊断和预后价值。通过定量实时聚合酶链反应（qRT-PCR）检测 88 例健康对照者和 90 例 NS 患者血清 miR-455-5p 水平。采用 Pearson 相关系数评估 miR-455-5p 与临床特征的相关性。采用受试者工作特征（ROC）曲线分析 miR-455-5p 的诊断评估。Kaplan-Meier 生存曲线和多因素 Cox 回归分析 miR-455-5p 在 NS 中的预后价值。与健康对照组相比，NS 患者血清 miR-455-5p 表达水平明显升高（<0.001），miR-455-5p 水平与白细胞计数（WBC）和其他临床特征呈正相关（<0.01）。ROC 曲线的 AUC 值为 0.895，提示 miR-455-5p 对 NS 具有诊断价值。生存分析表明，miR-455-5p 高表达的患者预后较差（log rank = 0.015），miR-455-5p 可能是 NS 的潜在预后标志物（HR = 3.454，95%CI = 1.165-10.234， = 0.025）。miR-455-5p 的表达能够区分 NS 与健康人群，高表达的 miR-455-5p 与 NS 患者预后不良相关。
