Ignotz R A, Massagué J
Department of Biochemistry, University of Massachusetts Medical School, Worcester 01605.
Cell. 1987 Oct 23;51(2):189-97. doi: 10.1016/0092-8674(87)90146-2.
Transforming growth factor-beta (TGF-beta) increases the incorporation of fibronectin and type I collagen into the extracellular matrix of fibroblasts and epithelial cells and enhances the attachment of thymocytes onto a fibronectin substratum. Investigation of the molecular basis for these effects showed that TGF-beta elevates specifically the expression of cell adhesion protein receptors. Treatment of cells with either form of TGF-beta, TGF-beta 1, or TGF-beta 2, increases the rate of receptor synthesis and the level of receptors on the cell surface. TGF-beta acts via two complementary mechanisms, elevation of receptor mRNA and faster kinetics of receptor beta subunit precursor to product conversion. The results show that the expression of cell adhesion receptors is susceptible to pretranslational and posttranslational regulation by factors that control cell morphology, proliferation, and differentiation such as TGF-beta.
转化生长因子-β（TGF-β）可增加纤连蛋白和I型胶原蛋白掺入成纤维细胞和上皮细胞的细胞外基质中，并增强胸腺细胞在纤连蛋白基质上的附着。对这些效应的分子基础的研究表明，TGF-β特异性地提高了细胞粘附蛋白受体的表达。用TGF-β的任何一种形式（TGF-β1或TGF-β2）处理细胞，均可增加受体合成速率和细胞表面受体水平。TGF-β通过两种互补机制发挥作用，即提高受体mRNA水平以及加快受体β亚基前体向产物转化的动力学。结果表明，细胞粘附受体的表达易受控制细胞形态、增殖和分化的因子（如TGF-β）的转录前和转录后调控。
