Thompson C B, Humphries E H, Carlson L M, Chen C L, Neiman P E
Fred Hutchinson Cancer Research Center, Seattle, Washington.
Cell. 1987 Nov 6;51(3):371-81. doi: 10.1016/0092-8674(87)90633-7.
Infection of 18-day embryonic bursal lymphocytes with a v-myc-containing retrovirus leads directly to a polyclonal proliferation of surface immunoglobulin-positive (slg+) cells in the bursa of Fabricius detected four weeks after hatching. These v-myc-expressing bursal cells repopulate the follicles of chemically ablated bursae more efficiently than total normal 18-day embryonic bursal cells. In contrast, comparable normal bursal cells lose the ability to repopulate follicles by four weeks. Bursal lymphocytes expressing either a retroviral v-myc or a c-myc gene deregulated by adjacent retroviral integration retain the ability of embryonic bursal lymphocytes to diversify their immunoglobulin light chain genes. These results suggest that retroviral deregulation of myc expression during avian B cell development induces outgrowth of a population of cells with the cardinal phenotypic characteristics of bursal stem cells.
用含v-myc的逆转录病毒感染18日龄胚胎法氏囊淋巴细胞，直接导致孵化后四周在法氏囊中检测到表面免疫球蛋白阳性（slg+）细胞的多克隆增殖。这些表达v-myc的法氏囊细胞比正常18日龄胚胎法氏囊细胞更有效地重新填充化学切除法氏囊的滤泡。相比之下，正常的法氏囊细胞在四周时就失去了重新填充滤泡的能力。表达逆转录病毒v-myc或因相邻逆转录病毒整合而失调的c-myc基因的法氏囊淋巴细胞保留了胚胎法氏囊淋巴细胞使免疫球蛋白轻链基因多样化的能力。这些结果表明，在禽类B细胞发育过程中，逆转录病毒对myc表达的失调诱导了一群具有法氏囊干细胞主要表型特征的细胞的生长。
