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miRNA-130a-5p 的下调表达通过靶向 PTP4A2 加剧肝癌进展。

Downregulated Expression of miRNA-130a-5p Aggravates Hepatoma Progression via Targeting PTP4A2.

机构信息

Department of Hepatobiliary and Pancreatic Surgery, The Third Affiliated Hospital of Soochow University, Jiangsu, China.

Department of Neurosurgery, Xinyi People's Hospital, Jiangsu, China.

出版信息

Comput Math Methods Med. 2021 Dec 28;2021:4439505. doi: 10.1155/2021/4439505. eCollection 2021.

DOI:10.1155/2021/4439505
PMID:34992672
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8727122/
Abstract

BACKGROUND

Hepatoma is a leading cause of death worldwide, with high metastasis and recurrence rates. The aberrant expression of miRNA-130a-5p is involved in the development and progression of various cancers. However, there are no studies investigating the role of miRNA-130a-5p in hepatoma. The present study is aimed at clarifying the functional role of miRNA-130a-5p in hepatoma progression.

METHODS

The expression levels of miRNA-130a-5p in hepatoma tissues and cell lines were detected by qRT-PCR assays. Bioinformatic analysis, gain-/loss-of-function experiments, and luciferase activity assays were conducted to verify whether miRNA-130a-5p is targeted by protein tyrosine phosphatase 4A2 (PTP4A2). The functions of miRNA-130a-5p and PTP4A2 in hepatoma were determined by cell proliferation assays.

RESULTS

The expression of miRNA-130a-5p was downregulated in hepatoma tissues and was related to poor prognosis. However, the expression level of PTP4A2 was contradictory to that of miRNA-130a-5p, and PTP4A2 upregulation could aggravate hepatoma progression. The ectopic overexpression of PTP4A2 promoted hepatoma cell proliferation in vitro, which could be reversed by miRNA-130a-5p.

CONCLUSIONS

Our study implies that miRNA-130a-5p, which is downregulated in hepatoma tissues, can suppress hepatoma cell proliferation via targeting PTP4A2.

摘要

背景

肝癌是全球主要的死亡原因之一，其转移和复发率较高。miRNA-130a-5p 的异常表达与多种癌症的发生和发展有关。然而，目前尚无研究探讨 miRNA-130a-5p 在肝癌中的作用。本研究旨在阐明 miRNA-130a-5p 在肝癌进展中的功能作用。

方法

通过 qRT-PCR 检测肝癌组织和细胞系中 miRNA-130a-5p 的表达水平。通过生物信息学分析、增益/缺失功能实验和荧光素酶活性测定验证 miRNA-130a-5p 是否为蛋白酪氨酸磷酸酶 4A2（PTP4A2）的靶基因。通过细胞增殖实验确定 miRNA-130a-5p 和 PTP4A2 在肝癌中的功能。

结果

miRNA-130a-5p 在肝癌组织中表达下调，与预后不良相关。然而，PTP4A2 的表达水平与 miRNA-130a-5p 相反，PTP4A2 的上调可加重肝癌的进展。PTP4A2 的异位过表达可促进肝癌细胞在体外的增殖，而 miRNA-130a-5p 可逆转这种作用。

结论

我们的研究表明，在肝癌组织中下调的 miRNA-130a-5p 可通过靶向 PTP4A2 抑制肝癌细胞的增殖。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f15a/8727122/843795d827ae/CMMM2021-4439505.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f15a/8727122/66a361299e6e/CMMM2021-4439505.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f15a/8727122/6033fff0c3e7/CMMM2021-4439505.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f15a/8727122/2067b8b67e38/CMMM2021-4439505.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f15a/8727122/843795d827ae/CMMM2021-4439505.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f15a/8727122/66a361299e6e/CMMM2021-4439505.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f15a/8727122/6033fff0c3e7/CMMM2021-4439505.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f15a/8727122/2067b8b67e38/CMMM2021-4439505.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f15a/8727122/843795d827ae/CMMM2021-4439505.004.jpg

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