Jia Xiaowei, Si Xuyang, Jia Yangyang, Zhang Hongyan, Tian Shijun, Li Wenjing, Zhang Ke, Pan Yanyun
College of Life Science, Hebei Agricultural University/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding, Hebei, China.
College of Agronomy, Hebei Agricultural University/State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory of Crop Growth Regulation of Hebei Province, Baoding, Hebei, China.
PeerJ. 2021 Dec 14;9:e12480. doi: 10.7717/peerj.12480. eCollection 2021.
The inositol phospholipid signaling system mediates plant growth, development, and responses to adverse conditions. Diacylglycerol kinase (DGK) is one of the key enzymes in the phosphoinositide-cycle (PI-cycle), which catalyzes the phosphorylation of diacylglycerol (DAG) to form phosphatidic acid (PA). To date, comprehensive genomic and functional analyses of DGKs have not been reported in wheat. In this study, 24 DGK gene family members from the wheat genome (TaDGKs) were identified and analyzed. Each putative protein was found to consist of a DGK catalytic domain and an accessory domain. The analyses of phylogenetic and gene structure analyses revealed that each TaDGK gene could be grouped into clusters I, II, or III. In each phylogenetic subgroup, the TaDGKs demonstrated high conservation of functional domains, for example, of gene structure and amino acid sequences. Four coding sequences were then cloned from Chinese Spring wheat. Expression analysis of these four genes revealed that each had a unique spatial and developmental expression pattern, indicating their functional diversification across wheat growth and development processes. Additionally, were also prominently up-regulated under salt and drought stresses, suggesting their possible roles in dealing with adverse environmental conditions. Further cis-regulatory elements analysis elucidated transcriptional regulation and potential biological functions. These results provide valuable information for understanding the putative functions of DGKs in wheat and support deeper functional analysis of this pivotal gene family. The 24 TaDGKs identified and analyzed in this study provide a strong foundation for further exploration of the biological function and regulatory mechanisms of in response to environmental stimuli.
肌醇磷脂信号系统介导植物的生长、发育以及对逆境的响应。二酰基甘油激酶（DGK）是磷酸肌醇循环（PI循环）中的关键酶之一，它催化二酰基甘油（DAG）磷酸化形成磷脂酸（PA）。迄今为止，尚未见关于小麦中DGK的全面基因组和功能分析的报道。在本研究中，从小麦基因组中鉴定并分析了24个DGK基因家族成员（TaDGKs）。发现每个推定的蛋白质都由一个DGK催化结构域和一个辅助结构域组成。系统发育和基因结构分析表明，每个TaDGK基因可分为I、II或III簇。在每个系统发育亚组中，TaDGKs在功能结构域方面表现出高度保守性，例如在基因结构和氨基酸序列方面。随后从中国春小麦中克隆了四个编码序列。对这四个基因的表达分析表明，每个基因都有独特的空间和发育表达模式，表明它们在小麦生长和发育过程中的功能多样化。此外，在盐胁迫和干旱胁迫下它们也显著上调，表明它们在应对逆境条件中可能发挥的作用。进一步的顺式调控元件分析阐明了转录调控和潜在的生物学功能。这些结果为了解DGKs在小麦中的推定功能提供了有价值的信息，并支持对这个关键基因家族进行更深入的功能分析。本研究中鉴定和分析的24个TaDGKs为进一步探索其响应环境刺激的生物学功能和调控机制奠定了坚实基础。
