McCarroll D R, Lothrop S A, Dolan M C, McDonald T P
Department of Pediatrics, University of Illinois College of Medicine at Peoria.
Exp Hematol. 1987 Nov;15(10):1060-7.
Canine von Willebrand factor (vWf) was compared to human vWf. Antisera raised against human vWf or canine vWf cross-reacted with both heterologous proteins and reactions of partial identity were seen using crossed immunoelectrophoresis. Similar patterns of multimerization were obtained for vWf from both canine and human sources using the enzyme-linked immunoelectrotransfer blot method. However, the canine protein displayed an altered electrophoretic mobility. The molecular weight of the vWf monomer was estimated by SDS-PAGE and found to be indistinguishable from that of human vWf monomer. Canine vWf is decreased in animals with clinically evident hypothyroidism and in heterozygous "carriers" of von Willebrand's disease (vWd) that display no clinical symptoms of vWd. Results expand the concept that von Willebrand's disease in dogs may be a useful model for study of vWd in humans, and that immunochemical methods established for studies of human vWf appear appropriate for studies of canine vWf.
对犬血管性血友病因子（vWf）与人类vWf进行了比较。用抗人类vWf或抗犬vWf制备的抗血清与两种异源蛋白均发生交叉反应，采用交叉免疫电泳可观察到部分相同的反应。使用酶联免疫电转移印迹法，从犬和人类来源获得的vWf具有相似的多聚体模式。然而，犬类蛋白表现出改变的电泳迁移率。通过SDS-PAGE估计vWf单体的分子量，发现其与人类vWf单体的分子量没有区别。在临床上明显甲状腺功能减退的动物以及没有血管性血友病（vWd）临床症状的vWd杂合“携带者”中，犬vWf水平降低。研究结果扩展了这样一个概念，即犬类血管性血友病可能是研究人类vWd的有用模型，并且为研究人类vWf建立的免疫化学方法似乎适用于犬类vWf的研究。
