Department of Matrix Medicine, Faculty of Medicine, Oita University, Yufu, Oita, 879-5593, Japan.
Division of Life Science Research, Research Promotion Institute, Oita University, Yufu, Oita, 879-5593, Japan.
Histochem Cell Biol. 2022 Feb;157(2):205-216. doi: 10.1007/s00418-021-02059-9. Epub 2022 Jan 7.
Collagen XIX is a nonfibrillar collagen that localizes in restricted tissues at very low amounts. A previous study on Col19a1 null mice revealed that collagen XIX is involved in esophageal muscle physiology and morphogenesis. Here, we use histological analysis to show that mice with a Col19a1 mutant lacking the NC3 domain and seven collagen triplets display abnormal transition of smooth to striated muscle in the abdominal segment of esophagus, and a widened esophagus with age. With two newly prepared antibodies, we analyzed the expression of collagen XIX in the mouse esophagus and show that collagen XIX colocalizes with α-smooth muscle actin. By immunoelectron microscopy, we confirmed the localization of collagen XIX in esophageal smooth muscle cells. Col19a1 mutant mice contained reduced levels of mutated Col19a1 mRNA. Interestingly, hepatocyte growth factor, which has an important role in esophageal striated muscle development, was reduced in the esophagus of the Col19a1 mutant mice. These findings suggest that collagen XIX may be critical for the function of esophageal smooth muscle cells as a scaffold for anteroposterior migration of esophagus-striated muscle cells.
胶原 19 是一种非纤维胶原，在含量非常低的特定组织中定位。之前对 Col19a1 基因敲除小鼠的研究表明，胶原 19 参与食管肌肉生理学和形态发生。在这里，我们使用组织学分析表明，缺乏 NC3 结构域和七个胶原三螺旋的 Col19a1 突变小鼠显示出食管腹段平滑肌到横纹肌的异常过渡，并且随着年龄的增长食管变宽。使用两种新制备的抗体，我们分析了小鼠食管中胶原 19 的表达，并表明胶原 19 与α-平滑肌肌动蛋白共定位。通过免疫电子显微镜，我们证实了胶原 19 在食管平滑肌细胞中的定位。Col19a1 突变小鼠中突变的 Col19a1 mRNA 水平降低。有趣的是，在 Col19a1 突变小鼠的食管中，对食管横纹肌发育具有重要作用的肝细胞生长因子减少。这些发现表明，胶原 19 可能是食管平滑肌细胞功能的关键，作为食管横纹肌细胞前后迁移的支架。
