Llosa Nicolas
Johns Hopkins University, Baltimore, MD, USA.
Methods Mol Biol. 2022;2435:139-156. doi: 10.1007/978-1-0716-2014-4_10.
With the modern success of immunotherapy from chimeric antigen receptor T-cell therapy to checkpoint blockade, an understanding of the tumor microenvironment has been more essential than ever. Knowing the key immune players in the tumor can provide the basis for studying cancer host immunity and interactions governing tumor tolerance. This endeavor can be approached in a step like manner-from a broad overview with immunohistochemistry to genomic expression analysis, followed by detailed functional analysis of each cell with multiparameter flow cytometry.
随着从嵌合抗原受体T细胞疗法到检查点阻断的免疫疗法在现代取得成功，对肿瘤微环境的理解比以往任何时候都更加重要。了解肿瘤中关键的免疫参与者可为研究癌症宿主免疫以及控制肿瘤耐受性的相互作用提供基础。这一工作可以按步骤进行——从通过免疫组织化学进行广泛概述，到基因组表达分析，再到使用多参数流式细胞术对每个细胞进行详细的功能分析。
