Department of Zoology, Faculty of Science, University of Gujrat, Hafiz Hayat Campus, Gujrat, 50700, Pakistan.
Department of Physics, Faculty of Science, University of Gujrat, Hafiz Hayat Campus, Gujrat, 50700, Pakistan.
Biol Trace Elem Res. 2022 Nov;200(11):4855-4864. doi: 10.1007/s12011-021-03067-5. Epub 2022 Jan 7.
Green synthesis of nanoparticles using plant-based extracts is momentously used in different fields of science because of their environment-friendly nature and cost-effectiveness. In the present study, silver nanoparticles were synthesized by using rice husk (non-toxic agricultural by-product) to determine their efficacy against aphid's (Sitobion avanae) mortality and antioxidant enzymes. UV-VIS spectroscopy of synthesized nanoparticles showed the maximum absorption peak at 440 nm, FTIR exhibited different peaks, and SEM confirmed the roughly spherical shape and 70-80 nm size of silver nanoparticles. Aphids were reared on wheat seedlings in the laboratory at 20-25 °C and 16:8 (light:dark) photoperiod. Insecticidal bioassays were conducted on aphids at three different concentrations (200 ppm, 400 ppm, 600 ppm) of nanoparticles for 2 days. Results showed the highest mortality of aphids being 93.3% at 600 ppm nanoparticle concentration after 2 days while the lowest mortality was observed at 200 ppm. Furthermore, the effect of silver nanoparticles on antioxidant enzymes was studied. Results of enzyme assays revealed that enzyme activities of catalase and glutathione-s-transferase increased in response to increased nanoparticle concentration. The current findings suggested that silver nanoparticles have probation for replacing commercially available insecticides for combating pests.
使用植物提取物进行纳米粒子的绿色合成因其环保性质和成本效益，在不同的科学领域得到了广泛应用。本研究使用稻壳（无毒的农业副产品）合成了银纳米粒子，以确定其对蚜虫（Sitobion avanae）死亡率和抗氧化酶的功效。合成纳米粒子的 UV-VIS 光谱在 440nm 处显示出最大吸收峰，FTIR 显示出不同的峰，SEM 证实了银纳米粒子大致呈球形且粒径为 70-80nm。蚜虫在 20-25°C 和 16:8（光照:黑暗）光周期的实验室小麦幼苗上饲养。在 2 天内，在三个不同浓度（200ppm、400ppm 和 600ppm）的纳米粒子下对蚜虫进行了杀虫生物测定。结果表明，在 2 天后，600ppm 纳米粒子浓度下蚜虫的死亡率最高，达到 93.3%，而在 200ppm 纳米粒子浓度下死亡率最低。此外，还研究了银纳米粒子对抗氧化酶的影响。酶活性测定结果表明，过氧化氢酶和谷胱甘肽-S-转移酶的酶活性随着纳米粒子浓度的增加而增加。目前的研究结果表明，银纳米粒子有望替代市售杀虫剂来防治害虫。
