College of Plant Science, Jilin University, No.5333 Xi'an Road, Changchun City, 130062, China.
Genome. 2022 Mar;65(3):153-164. doi: 10.1139/gen-2021-0043. Epub 2022 Jan 7.
Vegetable oil is one of the most important components of human nutrition. Soybean () is an important oil crop worldwide and contains rich unsaturated fatty acids. Diacylglycerol acyltransferase (DGAT) is a key rate-limiting enzyme in the Kennedy pathway from diacylglycerol (DAG) to triacylglycerol (TAG). In this study, we conducted further research using T transgenic soybean. A high-performance gas chromatography flame ionization detector showed that oleic acid (18:1) content and total fatty acid content of transgenic soybean were significantly higher than those of the wild type (WT). However, linoleic acid (18:2) was much lower than that in the WT. For further mechanistic studies, 20 differentially expressed proteins (DEPs) and 119 differentially expressed metabolites (DEMs) were identified between WT (JACK) and transgenic soybean mature seeds using proteomic and lipidomics analyses. Combined proteomic and lipidomics analyses showed that the upregulation of the key DEP (lipase GDSL domain-containing protein) in lipid transport and metabolic process induced an increase in the total fatty acid and 18:1 composition, but a decrease in the 18:2 composition of fatty acids. Our study provides new insights into the deep study of molecular mechanism underlying the enhancement of fatty acids in transgenic soybeans, especially oleic acid and total fatty acid, which are enhanced by over-expression of .
植物油是人类营养的重要组成部分之一。大豆是世界范围内重要的油料作物，含有丰富的不饱和脂肪酸。二酰基甘油酰基转移酶（DGAT）是从二酰基甘油（DAG）到三酰基甘油（TAG）的 Kennedy 途径中的关键限速酶。在这项研究中，我们使用 T 转基因大豆进行了进一步的研究。高性能气相色谱火焰离子化检测器显示，转基因大豆的油酸（18:1）含量和总脂肪酸含量明显高于野生型（WT）。然而，亚油酸（18:2）远低于 WT。为了进行进一步的机制研究，使用蛋白质组学和脂质组学分析，在 WT（JACK）和转基因大豆成熟种子之间鉴定出 20 个差异表达蛋白（DEPs）和 119 个差异表达代谢物（DEMs）。结合蛋白质组学和脂质组学分析表明，脂质转运和代谢过程中关键 DEP（脂肪酶 GDSL 结构域蛋白）的上调导致总脂肪酸和 18:1 组成增加，但脂肪酸的 18:2 组成减少。我们的研究为深入研究转基因大豆中脂肪酸增强的分子机制提供了新的见解，特别是通过过表达引起的油酸和总脂肪酸的增强。
