氧化还原活性生物炭促进厌氧氨氧化性能的可行性及机制。
The feasibility and mechanism of redox-active biochar for promoting anammox performance.
机构信息
State Key Laboratory of Pollution Control and Resources Reuse, Shanghai Institute of Pollution Control and Ecological Security, College of Environmental Science and Engineering, Tongji University, Siping Road, Shanghai 200092, PR China.
State Key Laboratory of Pollution Control and Resources Reuse, Shanghai Institute of Pollution Control and Ecological Security, College of Environmental Science and Engineering, Tongji University, Siping Road, Shanghai 200092, PR China.
出版信息
Sci Total Environ. 2022 Mar 25;814:152813. doi: 10.1016/j.scitotenv.2021.152813. Epub 2022 Jan 4.
Redox-active biochar has been regarded as an effective additive to promote heterotrophic denitrification, yet little is known about the feasibility of adding biochar for promoting anammox performance. In this study, we investigated the effects of different biochar doses (3-14 g/L; 1.5-7.1 g/g VSS) on anammox performance. Results showed that, in a short term (40 days), biochar could enhance anammox nitrogen removal rate (NRR) and nitrogen removal efficiency (NRE) by 0-18.0% and 0.2%-11.6%, respectively; this enhancement effect increased at 3-10 g biochar/L assays and reached a plateau at 10-14 g biochar/L assays. The optimal biochar dosage was identified to be 10 g/L (5.1 g/g VSS), with the NRR and NRE being 5.6%-18.0% and 4.0%-11.6% higher than those of the control, respectively. The highest specific anammox activity was simultaneously obtained at 10 g biochar/L assay, being 51% higher than that of the control. It revealed that biochar promoted the secretion of extracellular polymeric substances (increased by 30%-40% compared with that of the control) and increased the ratio of extracellular proteins to polysaccharides as well, directly enhancing the extracellular electron transfer capacity (ETC) of anammox biomass. The increased ETC of anammox biomass would further accelerate the metabolic activities of anammox bacteria, and promote the relative abundance of anammox bacteria, i.e., Ca. Brocadia was enriched by 5.8-12.6 folds than that of the control. These results demonstrate that biochar is feasible to enhance anammox activity and nitrogen removal performance, facilitating to a fast startup and enhanced nitrogen removal of anammox system.
氧化还原活性生物炭已被视为促进异养反硝化的有效添加剂，但对于添加生物炭来促进厌氧氨氧化性能的可行性知之甚少。在本研究中，我们考察了不同生物炭剂量（3-14 g/L；1.5-7.1 g/g VSS）对厌氧氨氧化性能的影响。结果表明，在短期（40 天）内，生物炭可以分别提高厌氧氨氧化氮去除率（NRR）和氮去除效率（NRE）0-18.0%和 0.2%-11.6%；在 3-10 g 生物炭/L 试验中，这种增强效果增加，在 10-14 g 生物炭/L 试验中达到平台。确定最佳生物炭剂量为 10 g/L（5.1 g/g VSS），NRR 和 NRE 分别比对照组高 5.6%-18.0%和 4.0%-11.6%。同时在 10 g 生物炭/L 试验中获得了最高的比厌氧氨氧化活性，比对照组高 51%。结果表明，生物炭促进了胞外聚合物的分泌（比对照组增加了 30%-40%），并增加了胞外蛋白与多糖的比例，直接增强了厌氧氨氧化生物量的胞外电子传递能力（ETC）。厌氧氨氧化生物量增加的 ETC 将进一步加速厌氧氨氧化菌的代谢活动，促进厌氧氨氧化菌的相对丰度，即 Ca。Brocadia 比对照组富集了 5.8-12.6 倍。这些结果表明，生物炭可以增强厌氧氨氧化活性和氮去除性能，有助于快速启动和增强厌氧氨氧化系统的氮去除。
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