Zinchenko V V, Saano A K, Babykin M M, Shestakov S V
Mol Gen Mikrobiol Virusol. 1987 Jul(7):11-3.
It has been shown that the plasmid R89S derivatives can be used as integrative vectors for bacteria in which the plasmid is unable to replicate autonomously. The chromosomal and plasmid fragments of phototrophic bacterium Rhodobacter sphaeroides have been cloned in plasmid pVZ365, a SmRKmR-derivative of R89S. The obtained recombinant plasmids were mobilized into R. sphaeroides cells by the I pcP-group conjugative plasmid R751. The frequencies of the SmR-transconjugants formation are 3.7.10(-5) to 5.6.10(-3) per recipient cell. The formation of the SmR-transconjugants has not been revealed in case of the plasmid pVZ365 mobilization. The recombinant molecules containing R. sphaeroides plasmid fragments have been shown to integrate into endogenous plasmids and form cointegrates with them.
已表明质粒R89S衍生物可用作细菌的整合载体，在这些细菌中该质粒无法自主复制。光合细菌球形红杆菌的染色体和质粒片段已克隆到质粒pVZ365中，pVZ365是R89S的SmRKmR衍生物。通过I pcP组接合质粒R751将获得的重组质粒转移到球形红杆菌细胞中。每个受体细胞形成SmR转接合子的频率为3.7×10^(-5)至5.6×10^(-3)。在质粒pVZ365转移的情况下未发现SmR转接合子的形成。已表明含有球形红杆菌质粒片段的重组分子可整合到内源质粒中并与之形成共整合体。
