Stekhin I N, Andrianov V M, Piruzian E S
Mol Gen Mikrobiol Virusol. 1986 Oct(10):31-3.
We have demonstrated the possibility of hybrid plasmid pSPA044 conjugative transfer from E. coli cells into different Rhizobium species. The bireplicon plasmid, constructed earlier in our laboratory, consisting of pBR325 and HindIII fragment 13 of the nopaline plasmid pTiC58 was mobilized for transfer by the helper plasmid pRK2013 with the frequency about 10(-4). We conclude the hybrid plasmid pSPA044 to be able to replicate stably in Rhizobiaceae cells.
我们已经证明了杂交质粒pSPA044从大肠杆菌细胞接合转移到不同根瘤菌物种中的可能性。我们实验室之前构建的双复制子质粒，由pBR325和胭脂碱型质粒pTiC58的HindIII片段13组成，通过辅助质粒pRK2013介导进行转移，转移频率约为10^(-4)。我们得出结论，杂交质粒pSPA044能够在根瘤菌科细胞中稳定复制。
