低温和振动研磨法制备的角豆（Ceratonia siliqua L.）豆荚粉中酚类物质的生物可给性。
Bioaccessibility of phenolics from carob (Ceratonia siliqua L.) pod powder prepared by cryogenic and vibratory grinding.
机构信息
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice 53210, Czech Republic.
Institute of Environmental and Chemical Engineering and Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice 53210, Czech Republic.
出版信息
Food Chem. 2022 May 30;377:131968. doi: 10.1016/j.foodchem.2021.131968. Epub 2021 Dec 29.
Carob pod powder prepared by cryogenic (CG) and vibratory grinding for 4 min (VG-4) and 8 min (VG-8) was evaluated for its antioxidant properties, and phenolic content. The bioaccessibility of phenolics was determined after the oral, gastric, and intestinal digestion phases in vitro. CG carob powder had a higher total phenolic content (6.46 mg gallic acid/g) and antioxidant capacities in terms of DPPH (15.60 mg Trolox/g) and ABTS (28.58 mg Trolox/g) assays. Quercitrin (44.54-64.68 μg/g) and cinnamic acid (27.48-31.40 μg/g) were the most abundant phenolics in all carob powder samples determined by liquid chromatography. The bioaccessibility of only ferulic acid (108%) had increased after digestion of the CG carob powder. Vibratory grinding (VG-4 and VG-8) improved the bioaccessibility of cinnamic acid (86-87%), vanillic acid (87-95%), quercitrin (33-34%), and naringenin (19-22%). A better bioaccessibility of phenolic constituents was observed for vibratory ground carob powder.
将低温（CG）和振动研磨 4 分钟（VG-4）和 8 分钟（VG-8）制备的角豆荚粉进行评估，以测定其抗氧化性能和酚类含量。在体外进行口服、胃和肠消化阶段后，测定了酚类物质的生物利用度。CG 角豆荚粉具有更高的总酚含量（6.46mg 没食子酸/g）和 DPPH（15.60mg Trolox/g）和 ABTS（28.58mg Trolox/g）测定的抗氧化能力。通过液相色谱法测定，所有角豆粉样品中含量最丰富的酚类物质为槲皮素（44.54-64.68μg/g）和肉桂酸（27.48-31.40μg/g）。仅 CG 角豆粉经消化后，阿魏酸（108%）的生物利用度增加。振动研磨（VG-4 和 VG-8）提高了肉桂酸（86-87%）、香草酸（87-95%）、槲皮苷（33-34%）和柚皮苷（19-22%）的生物利用度。振动研磨的角豆粉中观察到酚类成分具有更好的生物利用度。
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