• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

核-细胞骨架通讯影响胚胎干细胞中的 OCT4-染色质相互作用。

Nucleus-cytoskeleton communication impacts on OCT4-chromatin interactions in embryonic stem cells.

机构信息

Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), CONICET-Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, C1428EGA, Buenos Aires, Argentina.

Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EGA, Buenos Aires, Argentina.

出版信息

BMC Biol. 2022 Jan 7;20(1):6. doi: 10.1186/s12915-021-01207-w.

DOI:10.1186/s12915-021-01207-w
PMID:34996451
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8742348/
Abstract

BACKGROUND

The cytoskeleton is a key component of the system responsible for transmitting mechanical cues from the cellular environment to the nucleus, where they trigger downstream responses. This communication is particularly relevant in embryonic stem (ES) cells since forces can regulate cell fate and guide developmental processes. However, little is known regarding cytoskeleton organization in ES cells, and thus, relevant aspects of nuclear-cytoskeletal interactions remain elusive.

RESULTS

We explored the three-dimensional distribution of the cytoskeleton in live ES cells and show that these filaments affect the shape of the nucleus. Next, we evaluated if cytoskeletal components indirectly modulate the binding of the pluripotency transcription factor OCT4 to chromatin targets. We show that actin depolymerization triggers OCT4 binding to chromatin sites whereas vimentin disruption produces the opposite effect. In contrast to actin, vimentin contributes to the preservation of OCT4-chromatin interactions and, consequently, may have a pro-stemness role.

CONCLUSIONS

Our results suggest roles of components of the cytoskeleton in shaping the nucleus of ES cells, influencing the interactions of the transcription factor OCT4 with the chromatin and potentially affecting pluripotency and cell fate.

摘要

背景

细胞骨架是负责将细胞外环境的机械线索传递到细胞核的系统的关键组成部分，在细胞核中，它们引发下游反应。这种通讯在胚胎干细胞 (ES) 中尤为重要，因为力可以调节细胞命运并指导发育过程。然而，关于 ES 细胞中细胞骨架的组织知之甚少，因此，核-细胞骨架相互作用的相关方面仍然难以捉摸。

结果

我们探索了活 ES 细胞中细胞骨架的三维分布，表明这些纤维会影响细胞核的形状。接下来，我们评估了细胞骨架成分是否间接调节多潜能转录因子 OCT4 与染色质靶标的结合。我们表明，肌动蛋白解聚会触发 OCT4 与染色质位点的结合，而中间丝的破坏则会产生相反的效果。与肌动蛋白相反，中间丝有助于 OCT4-染色质相互作用的维持，因此可能具有促进干性的作用。

结论

我们的结果表明细胞骨架成分在塑造 ES 细胞的细胞核、影响转录因子 OCT4 与染色质的相互作用以及潜在影响多能性和细胞命运方面发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0553/8742348/c14cb5f3427e/12915_2021_1207_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0553/8742348/b346d07b898e/12915_2021_1207_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0553/8742348/167513ebf8be/12915_2021_1207_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0553/8742348/e1d42cb3c740/12915_2021_1207_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0553/8742348/5400d1e5c0c1/12915_2021_1207_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0553/8742348/c14cb5f3427e/12915_2021_1207_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0553/8742348/b346d07b898e/12915_2021_1207_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0553/8742348/167513ebf8be/12915_2021_1207_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0553/8742348/e1d42cb3c740/12915_2021_1207_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0553/8742348/5400d1e5c0c1/12915_2021_1207_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0553/8742348/c14cb5f3427e/12915_2021_1207_Fig5_HTML.jpg

相似文献

1
Nucleus-cytoskeleton communication impacts on OCT4-chromatin interactions in embryonic stem cells.核-细胞骨架通讯影响胚胎干细胞中的 OCT4-染色质相互作用。
BMC Biol. 2022 Jan 7;20(1):6. doi: 10.1186/s12915-021-01207-w.
2
Dynamical reorganization of the pluripotency transcription factors Oct4 and Sox2 during early differentiation of embryonic stem cells.胚胎干细胞早期分化过程中多能转录因子 Oct4 和 Sox2 的动态重组织。
Sci Rep. 2020 Mar 23;10(1):5195. doi: 10.1038/s41598-020-62235-0.
3
Kat6b Modulates Oct4 and Nanog Binding to Chromatin in Embryonic Stem Cells and Is Required for Efficient Neural Differentiation.Kat6b 调节胚胎干细胞中 Oct4 和 Nanog 与染色质的结合，并在有效的神经分化中起作用。
J Mol Biol. 2019 Mar 15;431(6):1148-1159. doi: 10.1016/j.jmb.2019.02.012. Epub 2019 Feb 18.
4
The pioneer factor OCT4 requires the chromatin remodeller BRG1 to support gene regulatory element function in mouse embryonic stem cells.先驱因子OCT4需要染色质重塑因子BRG1来支持小鼠胚胎干细胞中的基因调控元件功能。
Elife. 2017 Mar 13;6:e22631. doi: 10.7554/eLife.22631.
5
Differential role for transcription factor Oct4 nucleocytoplasmic dynamics in somatic cell reprogramming and self-renewal of embryonic stem cells.转录因子 Oct4 核质穿梭在体细胞重编程和胚胎干细胞自我更新中的差异作用。
J Biol Chem. 2013 May 24;288(21):15085-97. doi: 10.1074/jbc.M112.448837. Epub 2013 Apr 11.
6
A novel SALL4/OCT4 transcriptional feedback network for pluripotency of embryonic stem cells.一种新型的 SALL4/OCT4 转录反馈网络，用于胚胎干细胞的多能性。
PLoS One. 2010 May 21;5(5):e10766. doi: 10.1371/journal.pone.0010766.
7
HERV-H RNA is abundant in human embryonic stem cells and a precise marker for pluripotency.人类胚胎干细胞中含有丰富的 HERV-H RNA，是多能性的精确标志物。
Retrovirology. 2012 Dec 20;9:111. doi: 10.1186/1742-4690-9-111.
8
Conserved long noncoding RNAs transcriptionally regulated by Oct4 and Nanog modulate pluripotency in mouse embryonic stem cells.受 Oct4 和 Nanog 转录调控的保守长非编码 RNA 调节小鼠胚胎干细胞的多能性。
RNA. 2010 Feb;16(2):324-37. doi: 10.1261/rna.1441510. Epub 2009 Dec 21.
9
A role for mitotic bookmarking of SOX2 in pluripotency and differentiation.SOX2的有丝分裂标记在多能性和分化中的作用。
Genes Dev. 2016 Nov 15;30(22):2538-2550. doi: 10.1101/gad.289256.116. Epub 2016 Dec 5.
10
Oct4 dependence of chromatin structure within the extended Nanog locus in ES cells.胚胎干细胞中Nanog基因座扩展区域内染色质结构对Oct4的依赖性。
Genes Dev. 2008 Mar 1;22(5):575-80. doi: 10.1101/gad.1606308. Epub 2008 Feb 18.

引用本文的文献

1
Dynamic substrate topographies drive actin- and vimentin-mediated nuclear mechanoprotection events in human fibroblasts.动态底物拓扑结构驱动人成纤维细胞中肌动蛋白和波形蛋白介导的核机械保护事件。
BMC Biol. 2025 Apr 7;23(1):94. doi: 10.1186/s12915-025-02199-7.
2
Arp2/3 complex activity enables nuclear YAP for naïve pluripotency of human embryonic stem cells.Arp2/3 复合物的活性使核 YAP 能够维持人类胚胎干细胞的原始多能性。
Elife. 2024 Sep 25;13:e89725. doi: 10.7554/eLife.89725.
3
Bacterial Ribosomes Induce Plasticity in Mouse Adult Fibroblasts.

本文引用的文献

1
Membrane Tension Gates ERK-Mediated Regulation of Pluripotent Cell Fate.细胞膜张力门控 ERK 介导的多能性细胞命运调控。
Cell Stem Cell. 2021 Feb 4;28(2):273-284.e6. doi: 10.1016/j.stem.2020.10.018. Epub 2020 Nov 19.
2
Cell Surface Mechanics Gate Embryonic Stem Cell Differentiation.细胞膜表面力学调控胚胎干细胞分化。
Cell Stem Cell. 2021 Feb 4;28(2):209-216.e4. doi: 10.1016/j.stem.2020.10.017. Epub 2020 Nov 17.
3
Spatial organization and crosstalk of vimentin and actin stress fibers regulate the osteogenic differentiation of human adipose-derived stem cells.
细菌核糖体诱导成年小鼠成纤维细胞发生可塑性。
Cells. 2024 Jun 27;13(13):1116. doi: 10.3390/cells13131116.
4
From the membrane to the nucleus: mechanical signals and transcription regulation.从细胞膜到细胞核：机械信号与转录调控
Biophys Rev. 2023 Aug 2;15(4):671-683. doi: 10.1007/s12551-023-01103-3. eCollection 2023 Aug.
5
Genetic dissection of the pluripotent proteome through multi-omics data integration.通过多组学数据整合对多能蛋白质组进行遗传剖析。
Cell Genom. 2023 Mar 23;3(4):100283. doi: 10.1016/j.xgen.2023.100283. eCollection 2023 Apr 12.
6
Cluster-Assembled Zirconia Substrates Accelerate the Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells.簇组装氧化锆基底促进骨髓间充质干细胞的成骨分化。
Nanomaterials (Basel). 2023 Feb 22;13(5):801. doi: 10.3390/nano13050801.
7
Capturing Transitional Pluripotency through Proline Metabolism.通过脯氨酸代谢捕获过渡性多能性。
Cells. 2022 Jul 6;11(14):2125. doi: 10.3390/cells11142125.
细胞骨架蛋白中间丝和肌动蛋白应力纤维的空间组织与相互作用调控人脂肪干细胞的成骨分化。
FASEB J. 2021 Feb;35(2):e21175. doi: 10.1096/fj.202000378RR. Epub 2020 Nov 17.
4
Vimentin protects differentiating stem cells from stress.波形蛋白保护分化中的干细胞免受应激。
Sci Rep. 2020 Nov 11;10(1):19525. doi: 10.1038/s41598-020-76076-4.
5
Microtubules control nuclear shape and gene expression during early stages of hematopoietic differentiation.微管在造血分化早期控制核形状和基因表达。
EMBO J. 2020 Dec 1;39(23):e103957. doi: 10.15252/embj.2019103957. Epub 2020 Oct 22.
6
Vimentin Association with Nuclear Grooves in Normal MEF 3T3 Cells.波形蛋白与正常 MEF 3T3 细胞核槽的关联。
Int J Mol Sci. 2020 Oct 10;21(20):7478. doi: 10.3390/ijms21207478.
7
Abscission Couples Cell Division to Embryonic Stem Cell Fate.细胞分离将细胞分裂与胚胎干细胞命运联系起来。
Dev Cell. 2020 Oct 26;55(2):195-208.e5. doi: 10.1016/j.devcel.2020.09.001. Epub 2020 Sep 25.
8
Apparent stiffness of vimentin intermediate filaments in living cells and its relation with other cytoskeletal polymers.活细胞中中间丝角蛋白的表观僵硬与其与其他细胞骨架聚合物的关系。
Biochim Biophys Acta Mol Cell Res. 2020 Aug;1867(8):118726. doi: 10.1016/j.bbamcr.2020.118726. Epub 2020 Apr 19.
9
Dynamical reorganization of the pluripotency transcription factors Oct4 and Sox2 during early differentiation of embryonic stem cells.胚胎干细胞早期分化过程中多能转录因子 Oct4 和 Sox2 的动态重组织。
Sci Rep. 2020 Mar 23;10(1):5195. doi: 10.1038/s41598-020-62235-0.
10
Is It Time to Start Transitioning From 2D to 3D Cell Culture?是时候开始从二维细胞培养向三维细胞培养转变了吗？
Front Mol Biosci. 2020 Mar 6;7:33. doi: 10.3389/fmolb.2020.00033. eCollection 2020.