Djilali S, Parodi A L, Levy D
Laboratoire Immunogénétique INRA, Ecole Nationale Vétérinaire, Maisons Alfort, France.
Vet Immunol Immunopathol. 1987 Sep;16(1-2):151-4. doi: 10.1016/0165-2427(87)90181-4.
Bovine peripheral blood lymphocytes (PBL) were examined for their ability to bind wheat germ agglutinin (WGA). This lectin labelled 43.8% +/- 11.95 of bovine PBL, whereas peanut agglutinin (PNA), a T cell marker, bound 59.4% +/- 8.67 cells, and surface immunoglobulin (SLG)-bearing cells constituted 24.15% +/- 8.47 of PBL. After panning fractionation of B (Slg+) and T (PNA+) lymphocytes. WGA labelled 89 to 97% of the enriched T cell population (80/87% PNA+; 2-4% Slg+) but only 6 to 8% of the enriched B cell population (85-91% Slg+; 5-7% PNA+).
检测了牛外周血淋巴细胞(PBL)与麦胚凝集素(WGA)结合的能力。这种凝集素标记了43.8%±11.95的牛PBL,而作为T细胞标志物的花生凝集素(PNA)结合了59.4%±8.67的细胞,且带有表面免疫球蛋白(SLG)的细胞占PBL的24.15%±8.47。在对B(Slg+)和T(PNA+)淋巴细胞进行淘选分级分离后,WGA标记了89%至97%的富集T细胞群体(80/87% PNA+;2 - 4% Slg+),但仅标记了6%至8%的富集B细胞群体(85 - 91% Slg+;5 - 7% PNA+)。