Slesak B, Harłozińska-Szmyrka A, Frydecka I
Department of Tumor Immunology, Medical University, Wroclaw/Poland.
Folia Haematol Int Mag Klin Morphol Blutforsch. 1990;117(1):31-5.
The binding of five fluorescein-labelled lectins: peanut agglutinin (PNA), lentil agglutinin (LEN), soybean agglutinin (SBA), wheat germ agglutinin (WGA) and asparagus pea agglutinin (ASP) to human B-cell chronic lymphocytic leukaemia (B-CLL) and B lymphocytes of normal donors was studied. The specificity of the fluorescence was demonstrated by inhibition with appropriate saccharides. The proportion of B cells was estimated using anti-B cell monoclonal antibody. Both leukaemic and normal B cells showed the binding ability of all except of one (ASP) studied lectins. We have found the differences in surface carbohydrate patterns between B-CLL and normal B lymphocytes. B-CLL cells showed the considerably lower ability to bind SBA and slightly higher expression of PNA and LEN receptors in comparison to normal B cells. The analysis of WGA binding allowed for recognizing two groups of CLL patients: one with high and the second one with low WGA receptor expression. The double marker studies revealed that B cells could simultaneously react with anti-B cell monoclonal antibody and fluorochrome labelled lectins.
花生凝集素（PNA）、扁豆凝集素（LEN）、大豆凝集素（SBA）、麦胚凝集素（WGA）和四棱豆凝集素（ASP）与人类B细胞慢性淋巴细胞白血病（B-CLL）及正常供体B淋巴细胞的结合情况。通过用适当的糖类进行抑制来证明荧光的特异性。使用抗B细胞单克隆抗体估计B细胞的比例。白血病B细胞和正常B细胞均显示出除一种（ASP）研究的凝集素外所有凝集素的结合能力。我们发现B-CLL与正常B淋巴细胞在表面碳水化合物模式上存在差异。与正常B细胞相比，B-CLL细胞显示出结合SBA的能力明显较低，而PNA和LEN受体的表达略高。对WGA结合的分析使得能够识别两组CLL患者：一组WGA受体表达高，另一组表达低。双标记研究表明，B细胞可同时与抗B细胞单克隆抗体和荧光染料标记的凝集素发生反应。
