Varkila K, Silvennoinen O, Hurme M
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Acta Pathol Microbiol Immunol Scand C. 1987 Aug;95(4):141-8. doi: 10.1111/j.1699-0463.1987.tb00022.x.
The immuno-regulatory role of AsialoGM1+ murine NK cells in the induction of allogeneic cytotoxic T cell responses was studied in vivo and in vitro. Depletion of ASGM1+ cells from the (CBAxC57BL/6)F1 cells used for the foot-pad immunization of CBA mice greatly reduced the formation of allospecific CTLs. However, highly purified ASGM1+ cells were not efficient stimulators of alloCTLs in vivo by themselves. When the same genetic combination was used in alloCTL stimulation culture in vitro, ASGM1+ cells were seen to suppress the activation of the CTL response. The opposite roles of ASGM1+ cells in the alloCTL activation in vivo and in vitro were seen when both lymphoid cells (spleen cells or peripheral blood cells) and non-lymphoid cells (epidermal cells) were used as stimulators. The data presented in this paper suggest that during the alloCTL activation, ASGM1+ cells have an important enhancing role in vivo; but in vitro these cells suppress the antigen-presenting cells.
研究了去唾液酸GM1+小鼠自然杀伤细胞（NK细胞）在诱导同种异体细胞毒性T细胞反应中的免疫调节作用，研究包括体内和体外实验。用于足垫免疫CBA小鼠的（CBAxC57BL/6）F1细胞中的ASGM1+细胞被清除后，大大降低了同种特异性细胞毒性T淋巴细胞（CTL）的形成。然而，高度纯化的ASGM1+细胞本身在体内并非有效的同种CTL刺激剂。当在体外同种CTL刺激培养中使用相同的基因组合时，可以看到ASGM1+细胞抑制CTL反应的激活。当淋巴细胞（脾细胞或外周血细胞）和非淋巴细胞（表皮细胞）都用作刺激剂时，观察到ASGM1+细胞在体内和体外同种CTL激活中具有相反的作用。本文提供的数据表明，在同种CTL激活过程中，ASGM1+细胞在体内具有重要的增强作用；但在体外，这些细胞会抑制抗原呈递细胞。
