Silvennoinen O
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Immunology. 1988 Jul;64(3):495-500.
In the present study we investigated the accessory function of natural killer (NK) cells in PHA, anti-CD3 (OKT3) and solid-phase-bound anti-CD3-induced T-cell proliferative responses, as well as in allogeneic mixed lymphocyte reaction (MLR). Although NK cell-enriched Percoll fraction cells showed accessory activity in all of the studied T-cell responses, the accessory function in anti-CD3 and allogenic responses were always found to be strictly dependent on the proportions of contaminating monocytes (Mo) and dendritic cells (DC). Purified CD16+ NK cells were totally incapable of inducing T-cell proliferation in anti-CD3, solid-phase-bound anti-CD3 and in allogeneic MLR responses, whereas these NK cells could support PHA-induced T-cell proliferation, although to a very low extent. Growing evidence suggests an inhibitory role of NK cells in various immune responses, but in the studied T-cell responses NK cells did not suppress either Mo or DC accessory ability.
在本研究中，我们调查了自然杀伤（NK）细胞在PHA、抗CD3（OKT3）和固相结合抗CD3诱导的T细胞增殖反应以及同种异体混合淋巴细胞反应（MLR）中的辅助功能。尽管富含NK细胞的Percoll分层细胞在所有研究的T细胞反应中均显示出辅助活性，但抗CD3和同种异体反应中的辅助功能始终被发现严格依赖于污染的单核细胞（Mo）和树突状细胞（DC）的比例。纯化的CD16 + NK细胞在抗CD3、固相结合抗CD3和同种异体MLR反应中完全无法诱导T细胞增殖，而这些NK细胞可以支持PHA诱导的T细胞增殖，尽管程度非常低。越来越多的证据表明NK细胞在各种免疫反应中具有抑制作用，但在所研究的T细胞反应中，NK细胞既不抑制Mo也不抑制DC的辅助能力。
