College of Food Science and Technology, Henan University of Technology, Zhengzhou, China.
J Sci Food Agric. 2022 Aug 15;102(10):4025-4034. doi: 10.1002/jsfa.11751. Epub 2022 Jan 21.
High hydrostatic pressure (HHP) treatment has been used to alleviate the allergenicity of soybeans, but there are little data about the potential antigenicity of β-conglycinin after HHP treatment.
We examined the effects of HHP treatment on the antigenicity and structure of β-conglycinin. When the pressure was 300 and 400 MPa, HHP treatment reduced the immunoglobulin (Ig)G binding capacity of β-conglycinin, while its IgE binding capacity did not change significantly. After in vitro digestion, both the IgE and IgG binding of β-conglycinin was obviously inhibited after HHP treatment at 400 MPa and 60 °C, although its binding capacity with linear epitope antibodies increased. Moreover, HHP treatment changed the secondary structure of β-conglycinin, the content of α-helix and random coils increased, while the β-sheet and β-turn decreased. After HHP treatment, the conformational structure was unfolded so that a large number of hydrophobic regions were exposed.
HHP treatment alleviated the potential antigenicity of β-conglycinin by modifying its structure, which facilitated in vitro digestion and destroyed epitopes. This research provides a new insight into the mechanism of HHP treatment that affects the sensitization of soy protein allergens. © 2022 Society of Chemical Industry.
高压处理（HHP）已被用于减轻大豆的致敏性，但关于 HHP 处理后β-伴大豆球蛋白潜在抗原性的数据较少。
我们研究了 HHP 处理对β-伴大豆球蛋白的抗原性和结构的影响。当压力为 300 和 400 MPa 时，HHP 处理降低了β-伴大豆球蛋白的免疫球蛋白（Ig）G 结合能力，而其 IgE 结合能力没有明显变化。体外消化后，在 400 MPa 和 60°C 下进行 HHP 处理后，β-伴大豆球蛋白的 IgE 和 IgG 结合均明显受到抑制，尽管其与线性表位抗体的结合能力增加。此外，HHP 处理改变了β-伴大豆球蛋白的二级结构，α-螺旋和无规卷曲的含量增加，而β-折叠和β-转角减少。HHP 处理后，构象结构展开，暴露出大量疏水区。
通过改变结构，HHP 处理减轻了β-伴大豆球蛋白的潜在抗原性，促进了体外消化并破坏了表位。这项研究为 HHP 处理影响大豆蛋白过敏原致敏性的机制提供了新的见解。© 2022 英国化学学会。
