Hospital of Stomatology, Jilin University, Qinghua Road 1500, Changchun 130021, China.
Hospital of Stomatology, Jilin University, Qinghua Road 1500, Changchun 130021, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Apr 5;270:120836. doi: 10.1016/j.saa.2021.120836. Epub 2021 Dec 30.
The detection of intracellular nitroreductase (NTR) activity is important for the study of hypoxia in organisms. In the present study, a Rhodol-derived fluorescent chemosensor (Rhod-NO) was synthesized in a one-step procedure. Rhod-NO exhibits 110-fold fluorescence enhancement in the presence of NTR. Moreover, Rhod-NO demonstrates high NTR selectivity and sensitivity (LOD, 0.6 ng/mL). The mode of Rhod-NO binding to NTR was also revealed by molecular docking. In addition, the reaction and luminescence mechanisms were evaluated by MS and TDDFT theoretical calculations, respectively. Finally, Rhod-NO was successfully applied to monitor NTR production during Escherichia coli (E. coli) growth, and to visually analyze NTR production in malignant oral cancer cells under hypoxia. Thus, Rhod-NO represents a new molecular tool to further understanding of the biological function of NTR.
细胞内硝基还原酶（NTR）活性的检测对于研究生物中的缺氧现象非常重要。在本研究中，通过一步法合成了一种基于 Rhodol 的荧光化学传感器（Rhod-NO）。Rhod-NO 在存在 NTR 的情况下表现出 110 倍的荧光增强。此外，Rhod-NO 具有高的 NTR 选择性和灵敏度（LOD，0.6ng/mL）。通过分子对接揭示了 Rhod-NO 与 NTR 结合的模式。此外，通过 MS 和 TDDFT 理论计算分别评估了反应和发光机制。最后，成功地将 Rhod-NO 用于监测大肠杆菌（E. coli）生长过程中的 NTR 产生，并在缺氧条件下可视化分析恶性口腔癌细胞中的 NTR 产生。因此，Rhod-NO 代表了一种新的分子工具，可以进一步了解 NTR 的生物学功能。
