Post-Graduate Program in Food Science and Technology, Department of Food Engineering, Federal University of Paraíba, 58051-900, João Pessoa, Brazil.
Department of Gastronomy, Center for Technology and Regional Development, Federal University of Paraíba, 58058-600, João Pessoa, Brazil.
J Dairy Sci. 2022 Mar;105(3):1889-1899. doi: 10.3168/jds.2021-21110. Epub 2022 Jan 5.
We evaluated the performance of Limosilactobacillus mucosae CNPC007 as an autochthonous adjunct culture in the production of goat milk Greek-style yogurt. The techno-functional, physicochemical, and sensory characteristics of the control yogurt (containing only starter culture, CY) and the probiotic yogurt (with the probiotic strain added, PY) were assessed during 28 d of refrigerated storage. Furthermore, we determined the survival of the strain throughout the gastrointestinal tract under simulated conditions. The PY yogurt had a lower extent of proteolysis index and a higher depth of proteolysis index. These results indicate that the proteolytic enzymes of L. mucosae may have a possible action in PY. The PY formulation exhibited viscosity almost 1.5 times as high as CY over the refrigeration period, probably due to higher production of exopolysaccharides by the probiotic strain, which directly interferes with the microstructure, texture, and viscosity of the product. The PY formulation received higher scores for color, flavor, and global acceptance at 1 d of storage and higher texture scores at 28 d. The counts of L. mucosae remained high (>7 log cfu/g and >8.5 log cfu/g) throughout mouth-ileum digestion and storage, respectively, in PY. The autochthonous adjunct culture of L. mucosae CNPC007 can be used for production of a novel potentially probiotic goat yogurt without negatively affecting the general characteristics of the product quality, adding value associated with maintaining its functional potential.
我们评估了黏膜罗伊氏乳杆菌 CNPC007 作为一种土著共生培养物在生产山羊奶希腊式酸奶中的性能。在冷藏储存 28 天期间,评估了对照酸奶(仅含发酵剂,CY)和益生菌酸奶(添加益生菌菌株,PY)的工艺功能、物理化学和感官特性。此外,我们还在模拟条件下确定了菌株在整个胃肠道中的存活情况。PY 酸奶的蛋白水解指数较低,蛋白水解深度较高。这些结果表明黏膜罗伊氏乳杆菌的蛋白酶可能对 PY 有一定的作用。在冷藏期间,PY 配方的粘度几乎是 CY 的 1.5 倍,这可能是由于益生菌菌株产生了更多的胞外多糖,这直接影响了产品的微观结构、质地和粘度。在储存 1 天时,PY 配方在颜色、风味和整体可接受性方面获得了更高的评分,在 28 天时在质地方面获得了更高的评分。在 PY 中,黏膜罗伊氏乳杆菌的计数在口腔-回肠消化和储存过程中分别保持在较高水平(>7 log cfu/g 和>8.5 log cfu/g)。黏膜罗伊氏乳杆菌 CNPC007 的土著共生培养物可用于生产新型潜在益生菌山羊酸奶,而不会对产品质量的一般特性产生负面影响,增加了维持其功能潜力的附加值。