Acid secretion and cyclic nucleotide content of the guinea pig isolated stomach in the presence of the ionophore A23187.

The involvement of calcium ions in stimulus-secretion coupling in the gastric mucosa is uncertain. Acid secretion and mucosal cyclic nucleotide content of an isolated stomach preparation from the guinea-pig have been measured in the presence of the ionophore A2318 (Lilly) over the concentration range 10(-6) M both in the presence and absence of a phosphodiesterase inhibitor (ICI 631978 1 X 10(-4) M). The rate of acid secretion and cyclic nucleotide content were increased by ICI 63197 as expected but were unaltered by the ionophore. These results suggest that a change in intracellular calcium ion concentration does not alter acid secretion in this preparation.