Environmental Biotechnology and Genomics Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur, 440020, Maharashtra, India.
Arch Microbiol. 2022 Jan 9;204(2):132. doi: 10.1007/s00203-021-02737-1.
Pseudomonas simiae EGD-AQ6 is capable of utilizing chloroaromatic compound i.e., 2-4-D efficiently in its biofilm phenotype. The differential accumulation of intermediate 4-chlorocatechol rates were significant in planktonic and biofilm phenotypes, as well as in the increased biofilm adapted cell numbers. Interestingly, response surface analysis demonstrated the combined positive effects of 2-4-D degradation and 4-CCA accumulation rates and the gene expression profiles, with significant up-regulation of degradative and biofilm genes, and greater participation of pellicle genes in the biofilm phenotypes than their planktonic counterparts, thereby revealing a phenotype variation. It positively validated the physiological data. Furthermore, the sequence similarity of the 2-4-D catabolic and biofilm-forming proteins (pel ABCDEFG and pga ABCD), which are responsible for building carbohydrate rich extracellular matrix, were significant with the respective organisms. This is the first study, which endorses this strain to be unique in efficient chloro-aromatic degradation through phenotype variation, thereby proving a potential candidate in the improvement of bioremediation technologies.
绿脓假单胞菌 EGD-AQ6 能够在生物膜表型中有效地利用氯代芳烃化合物，即 2-4-D。在浮游生物和生物膜表型以及生物膜适应细胞数量增加方面，中间产物 4-氯儿茶酚的差异积累率非常显著。有趣的是，响应面分析表明 2-4-D 降解和 4-CCA 积累率以及基因表达谱的综合积极影响，降解和生物膜基因的显著上调，以及生物膜表型中比浮游生物表型中更多的菌毛基因参与，从而揭示了表型变异。它正面验证了生理数据。此外，负责构建富含碳水化合物的细胞外基质的 2-4-D 分解代谢和生物膜形成蛋白（pel ABCDEFG 和 pga ABCD）的序列相似性与相应的生物体非常相似。这是第一项研究，证明该菌株通过表型变异在高效氯代芳烃降解方面具有独特性，从而证明它是生物修复技术改进的潜在候选者。
