Doern G V, Chapin K C
Department of Clinical Microbiology, University of Massachusetts Medical Center, Worcester 01605.
Diagn Microbiol Infect Dis. 1987 Aug;7(4):269-72. doi: 10.1016/0732-8893(87)90143-x.
A total of 180 strains of Haemophilus influenzae and 119 strains of Haemophilus parainfluenzae were characterized with respect to biotype (i.e., production of indole, urease, and ornithine decarboxylase) using conventional biochemical methods and two commercially available biotyping systems: Trio-Tube Haemophilus system (Carr Microbiologicals) and the Rapid NH System (Inovative Diagnostic Systems). Concordance between the results of the Trio-Tube system and conventional biochemicals was achieved with 294 of the 299 test organisms (98.3%). With the Rapid NH System, concordance with the results of conventional biochemical tests was observed with 275 of the 299 tests strains (92.0%). One previously unrecognized biotype of H. parainfluenzae, designated biotype VIII, is described. Typical reactions of this biotype include indole production but no production of urease or ornithine decarboxylase.
三联管嗜血杆菌系统（卡尔微生物公司）和快速NH系统（创新诊断系统），对总共180株流感嗜血杆菌和119株副流感嗜血杆菌进行了生物型（即吲哚、脲酶和鸟氨酸脱羧酶的产生情况）鉴定。三联管系统与传统生化方法的结果在299株测试菌株中的294株（98.3%）上达成一致。对于快速NH系统，在299株测试菌株中的275株（92.0%）上观察到与传统生化测试结果一致。描述了一种先前未被识别的副流感嗜血杆菌生物型，命名为生物型VIII。该生物型的典型反应包括产生吲哚，但不产生脲酶或鸟氨酸脱羧酶。
