Kindler V, Thorens B, Vassalli P
Department of Pathology, University of Geneva, Centre Medical Universitaire, Switzerland.
Eur J Immunol. 1987 Oct;17(10):1511-4. doi: 10.1002/eji.1830171020.
Normal and irradiated mice were perfused with recombinant interleukin 3 (rIL3) and the number of early hemopoietic progenitors (CFU-S) was quantified in different organs. Normal mice perfused with rIL3 for 3 or 7 days showed a dramatic increase in the number of CFU-S in the spleen, liver and blood, while the bone marrow CFU-S number was slightly decreased. The total number of CFU-S per animal was only slightly increased; the major effect of rIL3 perfusion was thus on the distribution of CFU-S in the mice. Lethally irradiated mice injected with 5 X 10(4) syngeneic bone marrow cells and perfused with rIL3 were killed after 10 days. A significant increase in both the number and size of the splenic colonies was observed. The CFU-S content of these colonies, determined in a second set of irradiated mice, was increased by more than 25-fold, indicating that in conditions of hemopoietic depletion, rIL3 vastly increased the self-renewal capacity of early progenitors.
用重组白细胞介素3（rIL3）对正常小鼠和受辐照小鼠进行灌注，并对不同器官中早期造血祖细胞（CFU-S）的数量进行定量。用rIL3灌注3天或7天的正常小鼠，其脾脏、肝脏和血液中的CFU-S数量显著增加，而骨髓CFU-S数量略有减少。每只动物的CFU-S总数仅略有增加；因此，rIL3灌注的主要作用是影响CFU-S在小鼠体内的分布。对接受5×10⁴ 同基因骨髓细胞注射并灌注rIL3的致死性辐照小鼠，在10天后处死。观察到脾脏集落的数量和大小均显著增加。在另一组辐照小鼠中测定这些集落的CFU-S含量，发现增加了25倍以上，这表明在造血耗竭的情况下，rIL3极大地提高了早期祖细胞的自我更新能力。
