• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

LMCD1-AS1/miR-526b-3p/OSBPL5 轴促进非小细胞肺癌细胞的增殖、迁移和侵袭。

The LMCD1-AS1/miR-526b-3p/OSBPL5 axis promotes cell proliferation, migration and invasion in non-small cell lung cancer.

机构信息

Department of Thoracic Surgery, Shengli Oilfield Central Hospital, 31 Jinan Road, Dongying, 257034, Shandong, China.

出版信息

BMC Pulm Med. 2022 Jan 9;22(1):30. doi: 10.1186/s12890-022-01820-7.

DOI:10.1186/s12890-022-01820-7
PMID:35000595
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8744214/
Abstract

PURPOSE

To explore the specific role and regulatory mechanism of oxysterol binding protein like 5 (OSBPL5) in non-small cell lung cancer (NSCLC).

METHODS AND RESULTS

Quantitative real-time polymerase chain reaction (qRT-PCR) analysis demonstrated that OSBPL5 expression was notably elevated in NSCLC tissues and cell lines, and Kaplan-Meier analysis manifested that high OSBPL5 expression was closely related to the poor prognosis of NSCLC patients. Besides, according to the results from western blot analysis, cell counting kit-8, EdU and Transwell assays, knockdown of OSBPL5 suppressed NSCLC cell proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) process. Additionally, by performing qRT-PCR analysis, luciferase reporter and RNA pull-down assays, we verified that OSBPL5 was a downstream target of miR-526b-3p and long noncoding RNA (lncRNA) LMCD1-AS1 served as a sponge for miR-526b-3p. Moreover, from rescue assays, we observed that OSBPL5 overexpression offset LMCD1-AS1 knockdown-mediated inhibition in cell proliferation, migration, invasion and EMT in NSCLC.

CONCLUSIONS

This paper was the first to probe the molecular regulatory mechanism of OSBPL5 involving the LMCD1-AS1/miR-526b-3p axis in NSCLC and our results revealed that the LMCD1-AS1/miR-526b-3p/OSBPL5 axis facilitates NSCLC cell proliferation, migration, invasion and EMT, which may offer a novel therapeutic direction for NSCLC.

摘要

目的

探索氧化固醇结合蛋白样 5（OSBPL5）在非小细胞肺癌（NSCLC）中的具体作用和调控机制。

方法和结果

定量实时聚合酶链反应（qRT-PCR）分析表明，OSBPL5 在 NSCLC 组织和细胞系中表达明显上调，Kaplan-Meier 分析表明高 OSBPL5 表达与 NSCLC 患者的不良预后密切相关。此外，根据 Western blot 分析、细胞计数试剂盒-8（CCK-8）、EdU 和 Transwell 检测的结果，敲低 OSBPL5 抑制了 NSCLC 细胞的增殖、迁移、侵袭和上皮-间充质转化（EMT）过程。此外，通过 qRT-PCR 分析、荧光素酶报告基因和 RNA 下拉实验，我们验证了 OSBPL5 是 miR-526b-3p 的下游靶基因，长链非编码 RNA（lncRNA）LMCD1-AS1 作为 miR-526b-3p 的海绵。此外，从挽救实验中，我们观察到 OSBPL5 的过表达抵消了 LMCD1-AS1 敲低介导的 NSCLC 细胞增殖、迁移、侵袭和 EMT 抑制。

结论

本文首次探讨了 OSBPL5 涉及 NSCLC 中 LMCD1-AS1/miR-526b-3p 轴的分子调控机制，我们的结果表明，LMCD1-AS1/miR-526b-3p/OSBPL5 轴促进 NSCLC 细胞的增殖、迁移、侵袭和 EMT，这可能为 NSCLC 提供新的治疗方向。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d1b/8744214/701cc018df48/12890_2022_1820_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d1b/8744214/be6bd49c3003/12890_2022_1820_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d1b/8744214/977d82ad5f32/12890_2022_1820_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d1b/8744214/31df43f3c024/12890_2022_1820_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d1b/8744214/a580ac6d7288/12890_2022_1820_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d1b/8744214/701cc018df48/12890_2022_1820_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d1b/8744214/be6bd49c3003/12890_2022_1820_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d1b/8744214/977d82ad5f32/12890_2022_1820_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d1b/8744214/31df43f3c024/12890_2022_1820_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d1b/8744214/a580ac6d7288/12890_2022_1820_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d1b/8744214/701cc018df48/12890_2022_1820_Fig5_HTML.jpg

相似文献

1
The LMCD1-AS1/miR-526b-3p/OSBPL5 axis promotes cell proliferation, migration and invasion in non-small cell lung cancer.LMCD1-AS1/miR-526b-3p/OSBPL5 轴促进非小细胞肺癌细胞的增殖、迁移和侵袭。
BMC Pulm Med. 2022 Jan 9;22(1):30. doi: 10.1186/s12890-022-01820-7.
2
LMCD1-AS1 Facilitates Cell Proliferation and EMT by Sponging miR-873-3p in Cervical Cancer.LMCD1-AS1 通过海绵吸附 miR-873-3p 促进宫颈癌中的细胞增殖和 EMT。
Crit Rev Eukaryot Gene Expr. 2023;33(2):13-25. doi: 10.1615/CritRevEukaryotGeneExpr.2022042882.
3
Long Noncoding RNA HOXD-AS1 Promotes the Proliferation, Migration, and Invasion of Colorectal Cancer via the miR-526b-3p/CCND1 Axis.长链非编码 RNA HOXD-AS1 通过 miR-526b-3p/CCND1 轴促进结直肠癌的增殖、迁移和侵袭。
J Surg Res. 2020 Nov;255:525-535. doi: 10.1016/j.jss.2020.05.078. Epub 2020 Jul 5.
4
LncRNA NNT-AS1 promotes non-small cell lung cancer progression through regulating miR-22-3p/YAP1 axis.长链非编码 RNA NNT-AS1 通过调控 miR-22-3p/YAP1 轴促进非小细胞肺癌进展。
Thorac Cancer. 2020 Mar;11(3):549-560. doi: 10.1111/1759-7714.13280. Epub 2020 Jan 10.
5
Knockdown of lncRNA DLX6-AS1 inhibits cell proliferation, migration and invasion while promotes apoptosis by downregulating PRR11 expression and upregulating miR-144 in non-small cell lung cancer.敲低长链非编码 RNA DLX6-AS1 通过下调 PRR11 表达和上调 miR-144 抑制非小细胞肺癌细胞增殖、迁移和侵袭，促进细胞凋亡。
Biomed Pharmacother. 2019 Jan;109:1851-1859. doi: 10.1016/j.biopha.2018.09.151. Epub 2018 Nov 26.
6
LncRNA CCDC144NL-AS1 Serves as a Prognosis Biomarker for Non-small Cell Lung Cancer and Promotes Cellular Function by Targeting miR-490-3p.长链非编码RNA CCDC144NL-AS1作为非小细胞肺癌的预后生物标志物并通过靶向miR-490-3p促进细胞功能。
Mol Biotechnol. 2021 Oct;63(10):933-940. doi: 10.1007/s12033-021-00351-6. Epub 2021 Jun 11.
7
Downregulation of long non-coding RNA XIST inhibits cell proliferation, migration, invasion and EMT by regulating miR-212-3p/CBLL1 axis in non-small cell lung cancer cells.长链非编码 RNA XIST 的下调通过调节 miR-212-3p/CBLL1 轴抑制非小细胞肺癌细胞的增殖、迁移、侵袭和 EMT。
Eur Rev Med Pharmacol Sci. 2019 Oct;23(19):8391-8402. doi: 10.26355/eurrev_201910_19150.
8
E2F1-Induced Overexpression of Long Noncoding RNA SBF2-AS1 Promotes Non-Small-Cell Lung Cancer Metastasis Through Regulating miR-362-3p/GRB2 Axis.E2F1 诱导的长链非编码 RNA SBF2-AS1 过表达通过调节 miR-362-3p/GRB2 轴促进非小细胞肺癌转移。
DNA Cell Biol. 2020 Jul;39(7):1290-1298. doi: 10.1089/dna.2020.5426. Epub 2020 May 4.
9
LncRNA NCK1-AS1 promotes non-small cell lung cancer progression via regulating miR-512-5p/p21 axis.长链非编码 RNA NCK1-AS1 通过调控 miR-512-5p/p21 轴促进非小细胞肺癌进展。
Pathol Res Pract. 2020 Nov;216(11):153157. doi: 10.1016/j.prp.2020.153157. Epub 2020 Aug 9.
10
lncRNA PSMA3-AS1 promotes the progression of non-small cell lung cancer through targeting miR-17-5p/PD-L1.lncRNA PSMA3-AS1 通过靶向 miR-17-5p/PD-L1 促进非小细胞肺癌的进展。
Adv Clin Exp Med. 2021 Oct;30(10):1043-1050. doi: 10.17219/acem/138624.

引用本文的文献

1
The miRNA and PD-1/PD-L1 signaling axis: an arsenal of immunotherapeutic targets against lung cancer.微小RNA与程序性死亡蛋白1/程序性死亡配体1信号轴：肺癌免疫治疗靶点的宝库
Cell Death Discov. 2024 Sep 29;10(1):414. doi: 10.1038/s41420-024-02182-1.
2
miR-526b-5p/c-Myc/Foxp1 participates in recurrent spontaneous abortion by regulating the proliferation, migration, and invasion of trophoblasts.miR-526b-5p/c-Myc/Foxp1 通过调节滋养层细胞的增殖、迁移和侵袭参与复发性自然流产。
J Assist Reprod Genet. 2023 Jul;40(7):1559-1572. doi: 10.1007/s10815-023-02793-0. Epub 2023 Apr 13.
3
The expression, immune infiltration, prognosis, and experimental validation of OSBPL family genes in liver cancer.

本文引用的文献

1
Case Report: Dacomitinib Overcomes Osimertinib Resistance in NSCLC Patient Harboring L718Q Mutation: A Case Report.病例报告：达可替尼克服携带L718Q突变的非小细胞肺癌患者对奥希替尼的耐药性：一例病例报告。
Front Oncol. 2021 Dec 2;11:760097. doi: 10.3389/fonc.2021.760097. eCollection 2021.
2
Exosomal circPACRGL promotes progression of colorectal cancer via the miR-142-3p/miR-506-3p- TGF-β1 axis.外泌体 circPACRGL 通过 miR-142-3p/miR-506-3p-TGF-β1 轴促进结直肠癌的进展。
Mol Cancer. 2020 Jul 27;19(1):117. doi: 10.1186/s12943-020-01235-0.
3
Bmi-1-induced miR-27a and miR-155 promote tumor metastasis and chemoresistance by targeting RKIP in gastric cancer.
肝癌中 OSBPL 家族基因的表达、免疫浸润、预后和实验验证。
BMC Cancer. 2023 Mar 14;23(1):244. doi: 10.1186/s12885-023-10713-9.
Bmi-1 诱导的 miR-27a 和 miR-155 通过靶向胃癌中的 RKIP 促进肿瘤转移和化疗耐药性。
Mol Cancer. 2020 Jun 24;19(1):109. doi: 10.1186/s12943-020-01229-y.
4
ORP5 and ORP8: Sterol Sensors and Phospholipid Transfer Proteins at Membrane Contact Sites?ORP5 和 ORP8：膜接触位点的固醇传感器和磷脂转移蛋白？
Biomolecules. 2020 Jun 18;10(6):928. doi: 10.3390/biom10060928.
5
SP1-mediated upregulation of lncRNA LMCD1-AS1 functions a ceRNA for miR-106b-5p to facilitate osteosarcoma progression.SP1介导的lncRNA LMCD1-AS1上调作为miR-106b-5p的竞争性内源RNA发挥作用，促进骨肉瘤进展。
Biochem Biophys Res Commun. 2020 Jun 4;526(3):670-677. doi: 10.1016/j.bbrc.2020.03.151. Epub 2020 Apr 2.
6
CircRNA_0001449 disturbs phosphatidylinositol homeostasis and AKT activity by enhancing Osbpl5 translation in transient cerebral ischemia.环状 RNA_0001449 通过增强 Osbpl5 翻译在短暂性脑缺血中扰乱磷脂酰肌醇稳态和 AKT 活性。
Redox Biol. 2020 Jul;34:101459. doi: 10.1016/j.redox.2020.101459. Epub 2020 Feb 10.
7
Potential biomarkers screening to predict side effects of dexamethasone in different cancers.筛选潜在的生物标志物以预测地塞米松在不同癌症中的副作用。
Mol Genet Genomic Med. 2020 Apr;8(4):e1160. doi: 10.1002/mgg3.1160. Epub 2020 Feb 12.
8
LINC00958 Accelerates Cell Proliferation and Migration in Non-Small Cell Lung Cancer Through JNK/c-JUN Signaling.LINC00958通过JNK/c-JUN信号通路促进非小细胞肺癌细胞的增殖和迁移。
Hum Gene Ther Methods. 2019 Dec;30(6):226-234. doi: 10.1089/hgtb.2019.115.
9
Deciphering miRNAs' Action through miRNA Editing.通过 miRNA 编辑破译 miRNA 的作用。
Int J Mol Sci. 2019 Dec 11;20(24):6249. doi: 10.3390/ijms20246249.
10
RRM2 Regulated By LINC00667/miR-143-3p Signal Is Responsible For Non-Small Cell Lung Cancer Cell Progression.由LINC00667/miR-143-3p信号调控的RRM2负责非小细胞肺癌细胞的进展。
Onco Targets Ther. 2019 Nov 20;12:9927-9939. doi: 10.2147/OTT.S221339. eCollection 2019.