Master Bailing Liu's Tianchi Traumatology Inheritance Studio, Shenzhen Bailin Chinese Traditional Orthopaedic Hospital, Shenzhen, China.
Orthopedics Department, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen, China.
Bioengineered. 2022 Jan;13(1):1746-1757. doi: 10.1080/21655979.2021.2020394.
is a common traditional Chinese medicine (TCM) used in treating osteoarthritis (OA). The compatibility between effective components has now become a breakthrough in understanding the mechanism of TCM. This study aimed at determining the optimal compatibility and possible mechanism of for OA treatment. Results showed that the adhesion score of the OA group is higher than NC group, and showed a trend of down-regulation in the intervention group. The and IL-1β in joint fluid of the OA group was significantly increased compared to the sham operation group (NC group). Group G, I, and L exhibited significantly down-regulated , while groups C, F, I, K, and L exhibited reduced IL-1β. Joint adhesion, damage in cartilage, and synovial tissue was found in the OA model, cartilage tissue was found recovered in groups I, J, and L, and synovial tissue was recovered in group G, I, and L. Thus, group I and L were chosen for metabolite analysis, and indole-3-propionic acid was slightly up-regulated, while koeiginequinone A, prostaglandin H2, and 1-hydroxy-3-methoxy-10-methylacridonew were down-regulated in group I and L. According to functional analysis, the arachidonic acid (AA) metabolic pathway is enriched. Down-regulated expression of vital proteins in the AA metabolism pathway, such as PGE2 and 2 in group I and L were verified. In conclusion, at a compatibility of 0.03-μg/mg, 2.0-μg/mg, 20.0-μg/mg or 0.03-μg/mg, 2.0-μg/mg, 10.0-μg/mg, respectively, may be the optimal compatibility of .
是一种常用的中药（TCM），用于治疗骨关节炎（OA）。有效成分的配伍现在已成为理解中药机制的突破点。本研究旨在确定治疗 OA 的最佳配伍和可能的机制。结果表明，OA 组的粘连评分高于 NC 组，且干预组呈下调趋势。与假手术组（NC 组）相比，OA 组关节液中的 和 IL-1β显著增加。与 C、F、I、K 和 L 组相比，G、I 和 L 组 显著下调，而 IL-1β降低。OA 模型中发现关节粘连、软骨损伤和滑膜组织损伤，I、J 和 L 组中发现软骨组织恢复，G、I 和 L 组中发现滑膜组织恢复。因此，选择 I 和 L 组进行代谢物分析，发现吲哚-3-丙酸略有上调，而 I 和 L 组中的 koegienequinone A、前列腺素 H2 和 1-羟基-3-甲氧基-10-甲基吖啶酮下调。根据功能分析，发现花生四烯酸（AA）代谢途径富集。I 和 L 组中 AA 代谢途径中关键蛋白的表达下调，如 PGE2 和 2 得到验证。综上所述，在 0.03-μg/mg、2.0-μg/mg、20.0-μg/mg 或 0.03-μg/mg、2.0-μg/mg、10.0-μg/mg 的配伍下， 可能是 的最佳配伍。
