Papua New Guinea: 疟疾患者对群体按蚊的感染性。
Infectivity of Symptomatic Malaria Patients to Colony Mosquitoes in Papua New Guinea.
机构信息
Vector-borne Diseases Unit, Papua New Guinea Institute of Medical Research, Madang, Papua New Guinea.
Australian Institute of Tropical Health and Medicine, James Cook University, Smithfield, QLD, Australia.
出版信息
Front Cell Infect Microbiol. 2021 Dec 22;11:771233. doi: 10.3389/fcimb.2021.771233. eCollection 2021.
transmission from humans to mosquitoes is an understudied bottleneck in the transmission of malaria. Direct membrane feeding assays (DMFA) allow detailed malaria transmission studies from humans to mosquitoes. Especially for , which cannot be cultured long-term under laboratory conditions, implementation of DMFAs requires proximity to endemic areas. In this study, we investigated the infectivity of symptomatic infections to colony mosquitoes in Papua New Guinea (PNG). A total of 182 DMFAs were performed with venous blood collected from rapid diagnostic test (RDT) positive symptomatic malaria patients and subsequently analysed by light microscopy and quantitative real time polymerase chain reaction (qPCR). DMFAs resulted in mosquito infections in 20.9% (38/182) of cases. By light microscopy and qPCR, 10 - 11% of and 32 - 44% of positive individuals infected . Fifty-eight percent of and 15% of gametocytaemic infections infected .
从人类到蚊子的传播是疟疾传播中一个研究不足的瓶颈。直接膜喂养检测（DMFA）允许从人类到蚊子进行详细的疟疾传播研究。特别是对于那些不能在实验室条件下长期培养的，实施 DMFA 需要接近疟疾流行地区。在这项研究中，我们调查了 Papua New Guinea（PNG）有症状的疟疾病例对 种群蚊子的感染力。共进行了 182 次 DMFA，采集了快速诊断测试（RDT）阳性有症状疟疾病例的静脉血，随后通过光学显微镜和定量实时聚合酶链反应（qPCR）进行分析。DMFA 导致 20.9%（38/182）的病例发生蚊子感染。通过光学显微镜和 qPCR，10-11%的 和 32-44%的 阳性个体感染了 。58%的 和 15%的 配子血症感染了 。
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