Cole G A, Cole G A, Clements V K, Garcia E P, Ostrand-Rosenberg S
Department of Biology, University of Maryland Baltimore County, Catonsville 21228.
Proc Natl Acad Sci U S A. 1987 Dec;84(23):8613-7. doi: 10.1073/pnas.84.23.8613.
Murine A strain (KkDdLd) sarcoma I (SaI) tumor cells have been transfected with a cloned H-2Kb gene. The resulting clones (SKB clones) stably express high levels of a molecule that is serologically and biochemically indistinguishable from the H-2Kb antigen. SKB clones are not susceptible to cytotoxic T lymphocyte-mediated lysis by H-2Kb-specific bulk, cloned, or H-2Kb-restricted lymphocytic choriomeningitis virus-specific effectors. Survival times of A/J and B10.A mice challenged i.p. with the H-2Kb-expressing transfectants and the parental SaI cells are similar, suggesting that the presence of an allogeneic major histocompatibility complex class I antigen on the surface of this tumor line is insufficient for tumor rejection.
已用克隆的H-2Kb基因转染了小鼠A品系（KkDdLd）肉瘤I（SaI）肿瘤细胞。所得克隆（SKB克隆）稳定表达高水平的一种分子，该分子在血清学和生物化学上与H-2Kb抗原无法区分。SKB克隆不易被H-2Kb特异性大量克隆的或H-2Kb限制性淋巴细胞性脉络丛脑膜炎病毒特异性效应细胞介导的细胞毒性T淋巴细胞裂解。经腹腔注射表达H-2Kb的转染细胞和亲本SaI细胞攻击的A/J和B10.A小鼠的存活时间相似，这表明该肿瘤细胞系表面存在同种异体主要组织相容性复合体I类抗原不足以导致肿瘤排斥。
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