Henry S M, Baird S J, Woodfield D G
Department of Transfusion Medicine, Auckland Regional Blood Centre, Auckland Hospital, New Zealand.
Vox Sang. 1987;53(3):147-50. doi: 10.1111/j.1423-0410.1987.tb04939.x.
Previously described methods of phenotyping red cells sensitised with IgG using the indirect antiglobulin test required the dissociation of the coating protein. Based on an entirely different principle, Fab fragments of anti-human IgG (Fab anti-IgG) were used to block the antiglobulin binding sites on cell-bound IgG molecules, removing the necessity to dissociate them from the red cell. Fab anti-IgG was found to be effective in blocking interfering IgG on in vivo and in vitro IgG-sensitised red cells, permitting successful red cell phenotyping. Strongly IgG-sensitised samples which could not be fully neutralised by chloroquine diphosphate (CDP) or blocked with Fab anti-IgG alone could usually be phenotyped using a combination of both these methods. This new procedure may be of use in immunohaematology laboratories.
先前描述的使用间接抗球蛋白试验对用IgG致敏的红细胞进行表型分析的方法需要解离包被蛋白。基于完全不同的原理，抗人IgG的Fab片段（Fab抗IgG）被用于阻断细胞结合的IgG分子上的抗球蛋白结合位点，从而无需将它们从红细胞上解离下来。发现Fab抗IgG在体内和体外IgG致敏的红细胞上有效阻断干扰性IgG，从而能够成功地进行红细胞表型分析。不能被二磷酸氯喹（CDP）完全中和或仅用Fab抗IgG阻断的强IgG致敏样本通常可以使用这两种方法的组合进行表型分析。这一新方法可能在免疫血液学实验室中有用。
