Stensland Zachary C, Coleman Brianne M, Rihanek Marynette, Baxter Ryan M, Gottlieb Peter A, Hsieh Elena W Y, Sarapura Virginia D, Simmons Kimber M, Cambier John C, Smith Mia J
Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Barbara Davis Center for Diabetes, University of Colorado School of Medicine, Aurora, CO 80045, USA.
iScience. 2021 Dec 13;25(1):103626. doi: 10.1016/j.isci.2021.103626. eCollection 2022 Jan 21.
Autoimmune thyroid disease (AITD) is caused by aberrant activation of the immune system allowing autoreactive B and T cells to target the thyroid gland leading to disease. Although AITD is more frequently diagnosed in adults, children are also affected but rarely studied. Here, we performed phenotypic and functional characterization of peripheral blood immune cells from pediatric and adult-onset AITD patients and age-matched controls using mass cytometry. Major findings indicate that unlike adult-onset AITD patients, pediatric AITD patients exhibit a decrease in anergic B cells (B) and DN2 B cells and an increase in immature B cells compared to age-matched controls. These results indicate alterations in peripheral blood immune cells seen in pediatric-onset AITD could lead to rapid progression of disease. Hence, this study demonstrates diversity of AITD by showing differences in immune cell phenotypes and function based on age of onset, and may inform future therapies.
自身免疫性甲状腺疾病（AITD）是由免疫系统异常激活引起的，使得自身反应性B细胞和T细胞靶向甲状腺，从而导致疾病。虽然AITD在成年人中更常被诊断出来，但儿童也会受到影响，不过很少有相关研究。在这里，我们使用质谱流式细胞术对儿童和成人发病的AITD患者以及年龄匹配的对照者的外周血免疫细胞进行了表型和功能特征分析。主要研究结果表明，与成人发病的AITD患者不同，儿童AITD患者与年龄匹配的对照者相比，无反应性B细胞（B细胞）和DN2 B细胞减少，未成熟B细胞增加。这些结果表明，儿童发病的AITD中外周血免疫细胞的改变可能导致疾病的快速进展。因此，本研究通过显示基于发病年龄的免疫细胞表型和功能差异，证明了AITD的多样性，并可能为未来的治疗提供参考。
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