Gu Xiaoqiong, Sim Jean X Y, Lee Wei Lin, Cui Liang, Chan Yvonne F Z, Chang Ega Danu, Teh Yii Ean, Zhang An-Ni, Armas Federica, Chandra Franciscus, Chen Hongjie, Zhao Shijie, Lee Zhanyi, Thompson Janelle R, Ooi Eng Eong, Low Jenny G, Alm Eric J, Kalimuddin Shirin
Antimicrobial Resistance Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, 1 Create Way, Singapore 138602, Singapore.
Campus for Research Excellence and Technological Enterprise (CREATE), Singapore 138602, Singapore.
iScience. 2021 Dec 16;25(1):103644. doi: 10.1016/j.isci.2021.103644. eCollection 2022 Jan 21.
Antibiotic-associated diarrhea (AAD) affects a significant proportion of patients receiving antibiotics. We sought to understand if differences in the gut microbiome would influence the development of AAD. We administered a 3-day course of amoxicillin-clavulanate to 30 healthy adult volunteers, and analyzed their stool microbiome, using 16S rRNA gene sequencing, at baseline and up to 4 weeks post antibiotic administration. Lower levels of gut Ruminococcaceae were significantly and consistently observed from baseline until day 7 in participants who developed AAD. Overall, participants who developed AAD experienced a greater decrease in microbial diversity. The probability of AAD could be predicted based on qPCR-derived levels of at baseline. Our findings suggest that a lack of gut Ruminococcaceae influences development of AAD. Quantification of in stool prior to antibiotic administration may help identify patients at risk of AAD, and aid clinicians in devising individualized treatment regimens to minimize such adverse effects.
抗生素相关性腹泻（AAD）影响着很大一部分接受抗生素治疗的患者。我们试图了解肠道微生物群的差异是否会影响AAD的发生。我们对30名健康成年志愿者给予了为期3天的阿莫西林-克拉维酸疗程，并在基线以及抗生素给药后长达4周的时间里，使用16S rRNA基因测序分析了他们的粪便微生物群。在发生AAD的参与者中，从基线到第7天，肠道瘤胃球菌科的水平显著且持续较低。总体而言，发生AAD的参与者微生物多样性下降幅度更大。可以根据基线时qPCR得出的水平预测AAD的发生概率。我们的研究结果表明，肠道瘤胃球菌科的缺乏会影响AAD的发生。在抗生素给药前对粪便中的 进行定量分析，可能有助于识别有AAD风险的患者，并帮助临床医生制定个体化治疗方案，以尽量减少此类不良反应。
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