de Araújo Barbosa Vanessa, de Souza Cristian F, Pereira Alisson, Gatherer Derek, Brazil Reginaldo P, Bray Daniel P, Hamilton James G C
Laboratório Doenças Parasitárias, Instituto Oswaldo Cruz - Fiocruz, Pavilhão Arthur Neiva, Av. Brasil, 21040-360, Rio de Janeiro, RJ, Brazil.
Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, LA1 4YG, UK.
Curr Res Parasitol Vector Borne Dis. 2021;1:None. doi: 10.1016/j.crpvbd.2021.100044.
The sand fly is the main vector of in Brazil. Synthetic male-produced sex/aggregation pheromone co-located with micro-encapsulated λ-cyhalothrin in chicken sheds can significantly reduce canine infection and sand fly densities in a lure-and-kill strategy. In this study, we determined if insecticide-impregnated netting (IN) could replace insecticide residual spraying (IRS). We compared numbers of attracted and killed in experimental and real chicken sheds baited with pheromone and treated with a 1 m area of either insecticide spray or netting. First, we compared both treatments in experimental sheds to control mortality established from light trap captures. We then compared the long-term killing effect of insecticide spray and netting, without renewal, in experimental sheds over a period of 16 weeks. Finally, a longitudinal intervention study in real chicken sheds compared the numbers and proportions of collected and killed before and after application of both treatments. In Experiment 1, a higher proportion of males and females captured in IRS- and IN-treated sheds were dead at 24 h compared to controls ( < 0.05). No difference was found in the proportion of females killed in sheds treated with IN or IRS ( = 0.15). A slightly higher proportion of males were killed by IRS (100%) compared to IN (98.6%; < 0.05). In Experiment 2, IN- and IRS-treated traps were equally effective at killing females ( = 0.21) and males ( = 0.08). However, IRS killed a significantly higher proportion of females and males after 8 ( < 0.05) and 16 ( < 0.05) weeks. In Experiment 3, there was no significant difference between treatments in the proportion of females killed before ( = 0.88) or after ( = 0.29) or males killed before ( = 0.76) or after ( = 0.73) intervention. Overall, initially the IN was as effective as IRS at killing female and male in both experimental and real chicken sheds. However, the relative lethal effect of the IN deteriorated over time when stored under prevailing environmental conditions.
白蛉是巴西[某种疾病]的主要传播媒介。在鸡舍中，将合成的雄性产生的性/聚集信息素与微囊化的氯氟氰菊酯共同放置，采用诱杀策略可显著降低犬类感染率和白蛉密度。在本研究中，我们确定了浸药蚊帐（IN）是否可以替代杀虫剂残留喷洒（IRS）。我们比较了在使用信息素诱饵并分别用1平方米面积的杀虫剂喷雾或蚊帐处理的实验鸡舍和实际鸡舍中，被吸引和杀死的[某种昆虫]数量。首先，我们在实验鸡舍中比较了这两种处理方式与通过灯光诱捕确定的对照死亡率。然后，我们比较了在16周的时间内，实验鸡舍中未经更新的杀虫剂喷雾和蚊帐的长期杀灭效果。最后，在实际鸡舍中进行的一项纵向干预研究比较了两种处理方式应用前后捕获和杀死的[某种昆虫]数量及比例。在实验1中，与对照组相比，在接受IRS和IN处理的鸡舍中捕获的雄性和雌性[某种昆虫]在24小时时死亡比例更高（P<0.05）。在接受IN或IRS处理的鸡舍中杀死的雌性比例没有差异（P = 0.15）。与IN（98.6%）相比，IRS杀死的雄性比例略高（100%；P<0.05）。在实验2中，IN和IRS处理过的诱捕器在杀死雌性（P = 0.21）和雄性（P = 0.08）方面同样有效。然而，在8周（P<0.05）和16周（P<0.05）后，IRS杀死的雌性和雄性比例显著更高。在实验3中，处理前（P = 0.88）或处理后（P = 0.29）杀死的雌性比例以及处理前（P = 0.76）或处理后（P =
0.73）杀死的雄性比例在两种处理方式之间没有显著差异。总体而言，最初在实验鸡舍和实际鸡舍中，IN在杀死雌性和雄性[某种昆虫]方面与IRS一样有效。然而，在当时的环境条件下储存时，IN的相对致死效果随时间而恶化。
