Department of Rheumatology, The First Hospital of Shanxi Medical University, Taiyuan, China.
Department of Rheumatology, The Second Hospital of Shanxi Medical University, Taiyuan, China.
Front Immunol. 2021 Dec 23;12:768244. doi: 10.3389/fimmu.2021.768244. eCollection 2021.
Takayasu's arteritis (TA) is a type of primary large vessel vasculitis. Th1, Th17, and Tfh cells have been reported to be associated with TA relapse. However, the relationship between regulatory T cells (Tregs) and TA remains unclear.
To analyze the levels of circulating lymphocytes, especially Treg cells (CD4CD25FOXP3 T cells) and serum cytokines in TA patients and explore their relationship with their changes and TA disease activity.
A total of 57 TA patients and 43 sex- and age-matched healthy controls (HCs) were enrolled. According to NIH standards, 36 patients had active disease status. Flow cytometry combined with counting was used to detect the absolute numbers and ratios of Th1, Th2, Th17, and Treg cells in the peripheral blood of all the subjects. Magnetic bead-based multiplex immunoassay was used to detect cytokines.
Compared to HCs, the absolute number and proportion of peripheral Treg cells in TA patients was significantly decreased, while Th17 cells were significantly increased. Furthermore, compared to the inactive group, the TA active group had significantly increased levels of interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-α, but lower IL-10 levels. The absolute number of Th2 cells was negatively associated with platelet (PLT) and NIS scores in TA patients. The proportion of Th2 cells was negatively associated with the erythrocyte sedimentation rate in TA patients. After treatment, Treg cells were markedly increased.
There was a Th17-Treg cell imbalance with a significant reduction in peripheral Treg cells and an increase in Th17 cells in TA patients compared to the HCs. The levels of IL-6, IL-10, IL-17, and TNF-α appeared to be related to disease activity.
Takayasu 动脉炎（TA）是一种原发性大动脉血管炎。已有研究报道 Th1、Th17 和 Tfh 细胞与 TA 复发有关。然而，调节性 T 细胞（Treg）与 TA 之间的关系尚不清楚。
分析 TA 患者循环淋巴细胞，尤其是 Treg 细胞（CD4+CD25+FOXP3+T 细胞）和血清细胞因子的水平，并探讨其与变化的关系及其与 TA 疾病活动的关系。
共纳入 57 例 TA 患者和 43 名性别和年龄匹配的健康对照者（HCs）。根据 NIH 标准，36 例患者为活动期疾病状态。采用流式细胞术结合计数检测所有受试者外周血 Th1、Th2、Th17 和 Treg 细胞的绝对数和比例。采用磁珠基多重免疫分析检测细胞因子。
与 HCs 相比，TA 患者外周血 Treg 细胞的绝对数和比例明显降低，而 Th17 细胞明显增加。此外，与非活动组相比，TA 活动组的白细胞介素（IL）-6、IL-10 和肿瘤坏死因子（TNF）-α水平明显升高，而 IL-10 水平降低。Th2 细胞的绝对数与 TA 患者的血小板（PLT）和 NIS 评分呈负相关。Th2 细胞的比例与 TA 患者的红细胞沉降率呈负相关。治疗后，Treg 细胞明显增加。
与 HCs 相比，TA 患者存在 Th17-Treg 细胞失衡，外周 Treg 细胞减少，Th17 细胞增加。IL-6、IL-10、IL-17 和 TNF-α 水平似乎与疾病活动有关。
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