揭示 RT-LAMP 比色法 SARS-CoV-2 检测的机制，以提高检测可靠性。
Uncovering mechanisms of RT-LAMP colorimetric SARS-CoV-2 detection to improve assay reliability.
机构信息
Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
Quommni Technologies Limited, Hong Kong.
出版信息
Anal Methods. 2022 Jan 27;14(4):378-382. doi: 10.1039/d1ay01395e.
PMID:35006230
Abstract
Improved diagnostics are needed to manage the ongoing COVID-19 pandemic. In this study, we enhanced the color changes and sensitivity of colorimetric SARS-CoV-2 RT-LAMP assays based on triarylmethane dyes. We determined a mechanism for the color changes and obtained sensitivities of 10 RNA copies per microliter.
摘要
需要改进诊断方法来应对持续的 COVID-19 大流行。在这项研究中，我们基于三芳甲烷染料增强了比色 SARS-CoV-2 RT-LAMP 检测的颜色变化和灵敏度。我们确定了颜色变化的机制，并获得了每微升 10 个 RNA 拷贝的灵敏度。
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