Centro de Biotecnología-FEMSA, Tecnologico de Monterrey, CP 64849, Monterrey, NL, Mexico.
Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Laboratorio de Fisiología Molecular y Estructural, 66455, San Nicolás de los Garza, NL, Mexico and Alfa Medical Center, Guadalupe, CP 67100, NL, Mexico.
Anal Methods. 2021 Jan 21;13(2):169-178. doi: 10.1039/d0ay01658f.
We demonstrate a loop-mediated isothermal amplification (LAMP) method to detect and amplify SARS-CoV-2 genetic sequences using a set of in-house designed initiators that target regions encoding the N protein. We were able to detect and amplify SARS-CoV-2 nucleic acids in the range of 62 to 2 × 105 DNA copies by this straightforward method. Using synthetic SARS-CoV-2 samples and RNA extracts from patients, we demonstrate that colorimetric LAMP is a quantitative method comparable in diagnostic performance to RT-qPCR (i.e., sensitivity of 92.85% and specificity of 81.25% in a set of 44 RNA extracts from patients analyzed in a hospital setting).
我们展示了一种环介导等温扩增（LAMP）方法，该方法使用一组内部设计的启动子来检测和扩增编码 N 蛋白的区域中的 SARS-CoV-2 遗传序列。通过这种简单的方法，我们能够在 62 到 2×105 DNA 拷贝的范围内检测和扩增 SARS-CoV-2 核酸。使用合成的 SARS-CoV-2 样本和来自患者的 RNA 提取物，我们证明比色 LAMP 是一种与 RT-qPCR 相当的定量方法（即在医院环境中分析的 44 份 RNA 提取物中，灵敏度为 92.85%，特异性为 81.25%）。
