Applied Electrochemistry Division, Materials Science and Technology Research Institute (INTEMA), CONICET-University of Mar del Plata, Colon 10850, Mar del Plata 7600, Argentina.
Salemme Implants, Catamarca 2046, Mar del Plata 7600, Argentina.
ACS Appl Bio Mater. 2021 Sep 20;4(9):7123-7133. doi: 10.1021/acsabm.1c00735. Epub 2021 Aug 31.
Biodegradable materials based on magnesium alloys have a huge potential for bone fracture fixation devices due to their adequate mechanical properties and biocompatibility. However, their fast degradation and the consequent liberation of hydrogen gas at the initial stages of implantation is the major limitation for their use. In this study, the AZ91D magnesium alloy was surface treated by an environment-friendly, nontoxic, and low-cost anodizing process and the early in vivo response was studied in a rat transcortical model. Adequate maturation of woven bone around implants-detected at day 7 post implantation-to lamellar bone was observed from day 15. Lamellar bone after 15 and 30 days of implantation presented similar volume, mineralization pattern, mineral to protein content, and estimated bone maturity between anodized AZ91D and polylactic acid (control) implants. Histology observation showed neither release of hydrogen bubbles in the region closed to the anodized AZ91D implant nor systemic effects on liver, kidney, and spleen. Thus, anodizing of AZ91D in the conditions stated here induced an adequate short-term in vivo response, which postulates their use as potential biodegradable fracture fixation devices for bone healing.
基于镁合金的可生物降解材料因其足够的机械性能和生物相容性，在骨折固定装置方面具有巨大的潜力。然而，其在植入初期快速降解并随之产生大量氢气是限制其应用的主要原因。在这项研究中，采用环保、无毒且低成本的阳极氧化处理方法对 AZ91D 镁合金进行表面处理，并在大鼠皮质骨模型中研究了其早期的体内反应。植入后第 7 天即可观察到在植入物周围有足够成熟的编织骨形成-植入后第 15 天即可观察到板层骨，植入后第 15 天和第 30 天的板层骨在体积、矿化模式、矿物质与蛋白质含量以及估计的骨成熟度方面均无明显差异，且阳极氧化 AZ91D 与聚乳酸（对照）植入物之间的差异无统计学意义。组织学观察显示，在阳极氧化 AZ91D 植入物附近区域未观察到氢气泡的释放，也未对肝、肾和脾产生全身影响。因此，在此条件下对 AZ91D 进行阳极氧化处理可引起适当的短期体内反应，这为其作为潜在的可生物降解骨折固定装置用于骨愈合提供了依据。
