一种来自非洲鼓腹咝蝰（Bitis arietans）毒液的类半胱氨酸蛋白酶抑制剂。
A cystatin-like cysteine proteinase inhibitor from venom of the African puff adder (Bitis arietans).
作者信息
Evans H J, Barrett A J
机构信息
Biochemistry Department, Strangeways Laboratory, Cambridge, U.K.
出版信息
Biochem J. 1987 Sep 15;246(3):795-7. doi: 10.1042/bj2460795.
Abstract
Venoms from eight snakes have been screened for inhibitory activity against papain, strong activity being found in that of the African puff adder, Bitis arietans. The inhibitor from B. arietans venom has been purified by affinity chromatography on carboxymethyl-papain-Sepharose and ion-exchange chromatography. The inhibitor had an apparent Mr of 13,000 in SDS/polyacrylamide gel electrophoresis, and pI value of 6.5 (major component) or 6.3 (minor component). Values of Ki for the inhibition of papain, cathepsin B and dipeptidyl peptidase I were 0.10, 2.7 and 0.23 nM, respectively; chicken calpain was not inhibited.
摘要
对八种蛇的毒液进行了针对木瓜蛋白酶抑制活性的筛选，发现非洲鼓腹咝蝰（Bitis arietans）的毒液具有很强的活性。通过羧甲基木瓜蛋白酶-琼脂糖亲和层析和离子交换层析对来自鼓腹咝蝰毒液的抑制剂进行了纯化。该抑制剂在SDS/聚丙烯酰胺凝胶电泳中的表观分子量为13,000，等电点值为6.5（主要成分）或6.3（次要成分）。其对木瓜蛋白酶、组织蛋白酶B和二肽基肽酶I抑制作用的Ki值分别为0.10、2.7和0.23 nM；对鸡钙蛋白酶没有抑制作用。
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