Instituto de Acuicultura, Departamento de Microbiología y Parasitología, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
J Fish Dis. 2022 Apr;45(4):561-568. doi: 10.1111/jfd.13581. Epub 2022 Jan 10.
Nervous necrosis virus (NNV) is a hazardous aquatic pathogen, distributed worldwide and in a wide range of temperatures. Viral persistence in water has been demonstrated to be affected by different factors, such as temperature, UV, or biological load. In this study, we have investigated the viability of NNV strains in low- and high-salinity seawater (LS and HS, respectively) both in laboratory and aquarium conditions, at different storage temperatures, and for comparative purposes, in culture medium. Our results showed the highest NNV viability in seawater at 15°C and as temperature increased, a drop in viral persistence was observed. Additionally, survival at 15 and 30°C was strongly affected by increasing salt content, while no differences were observed between LS and HS groups at 20 and 25°C. The results of the incubation under aquarium conditions indicated that the effect of UV light and oxygen exposure accelerate the inactivation of infective particles. According to previous studies, NNV persistence in cell culture medium was higher than in seawater, and as observed in the latter, increasing incubation temperatures led to a decrease in viral survival.
神经坏死病毒（NNV）是一种危险的水生病原体，分布于全球范围内的各种温度环境中。病毒在水中的持续存在受到多种因素的影响，如温度、紫外线或生物负荷。在这项研究中，我们在实验室和水族箱条件下，在不同的储存温度下，以及为了比较目的，在培养基中，研究了 NNV 株在低盐度和高盐度海水中（分别为 LS 和 HS）的存活能力。结果表明，15°C 时海水中的 NNV 存活能力最高，随着温度的升高，病毒的持续存在能力下降。此外，在 15°C 和 30°C 下，盐度的增加强烈影响了病毒的存活能力，而在 20°C 和 25°C 下，LS 和 HS 组之间没有观察到差异。在水族箱条件下的孵育结果表明，紫外线和氧气暴露的影响会加速感染性颗粒的失活。根据之前的研究，NNV 在细胞培养基中的存活能力高于海水中的存活能力，并且与在海水中观察到的情况一样，随着孵育温度的升高，病毒的存活能力下降。
