Environmental Technology, Wageningen University & Research, P.O. Box 17, 6700, EV Wageningen, the Netherlands.
Environmental Technology, Wageningen University & Research, P.O. Box 17, 6700, EV Wageningen, the Netherlands; Laboratory of Microbiology, Wageningen University & Research, P.O. Box 8033, 6700, EH Wageningen, the Netherlands.
Environ Pollut. 2022 Apr 15;299:118807. doi: 10.1016/j.envpol.2022.118807. Epub 2022 Jan 7.
Groundwater quality is crucial for drinking water production, but groundwater resources are increasingly threatened by contamination with pesticides. As pesticides often occur at micropollutant concentrations, they are unattractive carbon sources for microorganisms and typically remain recalcitrant. Exploring microbial communities in aquifers used for drinking water production is an essential first step towards understanding the fate of micropollutants in groundwater. In this study, we investigated the interaction between groundwater geochemistry, pesticide presence, and microbial communities in an aquifer used for drinking water production. Two groundwater monitoring wells in The Netherlands were sampled in 2014, 2015, and 2016. In both wells, water was sampled from five discrete depths ranging from 13 to 54 m and was analyzed for geochemical parameters, pesticide concentrations and microbial community composition using 16S rRNA gene sequencing and qPCR. Groundwater geochemistry was stable throughout the study period and pesticides were heterogeneously distributed at low concentrations (μg L range). Microbial community composition was also stable throughout the sampling period. Integration of a unique dataset of chemical and microbial data showed that geochemical parameters and to a lesser extent pesticides exerted selective pressure on microbial communities. Microbial communities in both wells showed similar composition in the deeper aquifer, where pumping results in horizontal flow. This study provides insight into groundwater parameters that shape microbial community composition. This information can contribute to the future implementation of remediation technologies to guarantee safe drinking water production.
地下水质量对于饮用水生产至关重要，但地下水资源正日益受到农药污染的威胁。由于农药通常以微量污染物的浓度存在，它们对微生物来说不是有吸引力的碳源，通常仍然难以降解。探索用于饮用水生产的含水层中的微生物群落是了解地下水中小分子污染物命运的重要第一步。在这项研究中，我们研究了用于饮用水生产的含水层中的地下水地球化学、农药存在与微生物群落之间的相互作用。2014 年、2015 年和 2016 年，在荷兰的两口地下水监测井中进行了采样。在两口井中，从 13 到 54 米的五个不同深度采集水样，并使用 16S rRNA 基因测序和 qPCR 分析地球化学参数、农药浓度和微生物群落组成。整个研究期间，地下水地球化学性质稳定，农药浓度低且分布不均（μg L 范围）。微生物群落组成在整个采样期间也保持稳定。对化学和微生物数据的独特数据集进行整合表明，地球化学参数以及在较小程度上的农药对微生物群落施加了选择性压力。两口井中的微生物群落在深层含水层中具有相似的组成，在该层中，抽水导致水平流动。本研究深入了解了塑造微生物群落组成的地下水参数。这些信息可以为未来实施修复技术以保证安全饮用水生产做出贡献。
