Hofmann B, Fugger L, Ryder L P, Gaub J, Odum N, Platz P, Gerstoft J, Svejgaard A
Department of Clinical Immunology, University Hospital (Rigshospitalet) and State Serum Institute, Copenhagen, Denmark.
Cancer Detect Prev Suppl. 1987;1:619-26.
We studied the effects of exogenous T cell growth factor (TCGF) (= interleukin-2) and indomethacine on the lymphocyte transformation response in vitro to allogeneic cells, mitogens, and antigens in AIDS patients, those with AIDS-related complex (ARC), and in healthy controls. While low amounts of TCGF reduced the response of peripheral blood mononuclear cells (PBMC) to allogeneic cells in both healthy controls and AIDS patients, large amounts of TCGF augmented the response in both groups, although the response of the patients' cells were still subnormal. By depleting the PBMC for either CD4-positive or CD8-positive cells, the effect of TCGF on suboptimally mitogen-stimulated PBMC from controls was shown to be due to an increased response in both the CD4-positive and the CD8-positive cells. In contrast, with patient cells, TCGF only increased the response of the CD4-positive cells, while that of the CD8-positive cells was largely unchanged. Thus, the lack of normalization of the mitogen response of patient cells upon addition of TCGF may be largely due to unresponsiveness of CD8-positive cells to TCGF. This observation further supports the idea that CD8-positive cells are abnormal. To investigate the role of the inhibitor of TCGF production, PGE2, in AIDS, indomethacine was added to cultures of mitogen-stimulated PBMC from controls and patients. No differences were found between the three groups: the responses to PHA were slightly increased and those to Con A were unchanged.
我们研究了外源性T细胞生长因子（TCGF）（即白细胞介素-2）和消炎痛对艾滋病患者、艾滋病相关综合征（ARC）患者以及健康对照者外周血单个核细胞（PBMC）体外对同种异体细胞、有丝分裂原和抗原的淋巴细胞转化反应的影响。虽然低剂量的TCGF降低了健康对照者和艾滋病患者外周血单个核细胞对同种异体细胞的反应，但高剂量的TCGF增强了两组的反应，尽管患者细胞的反应仍低于正常水平。通过去除PBMC中的CD4阳性或CD8阳性细胞，发现TCGF对来自对照者的亚最佳有丝分裂原刺激的PBMC的作用是由于CD4阳性和CD8阳性细胞的反应均增加。相比之下，对于患者细胞，TCGF仅增加了CD4阳性细胞的反应，而CD8阳性细胞的反应基本未变。因此，添加TCGF后患者细胞有丝分裂原反应未能恢复正常，可能在很大程度上是由于CD8阳性细胞对TCGF无反应。这一观察结果进一步支持了CD8阳性细胞异常的观点。为了研究TCGF产生的抑制剂前列腺素E2（PGE2）在艾滋病中的作用，将消炎痛添加到来自对照者和患者的有丝分裂原刺激的PBMC培养物中。三组之间未发现差异：对PHA的反应略有增加，对刀豆蛋白A的反应未变。
