高能激光治疗悬韧带分支损伤模型中的组织愈合的组织学研究。

Histological tissue healing following high-power laser treatment in a model of suspensory ligament branch injury.

机构信息

Department of Virology, Parasitology and Immunology, Research group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium.

Tierklinik Lüsche GmbH, Bakum, Germany.

出版信息

Equine Vet J. 2022 Nov;54(6):1114-1122. doi: 10.1111/evj.13556. Epub 2022 Jan 20.

DOI:10.1111/evj.13556

PMID:35008124

Abstract

BACKGROUND

High-power laser therapy gained popularity recently as a regenerative treatment for tendinitis and desmitis in the horse. However, studies evaluating the effects of laser therapy on tissue repair at the histological level in large mammals are lacking.

OBJECTIVES

To evaluate the effects of high-power laser therapy on suspensory desmitis healing, using a model of suspensory ligament branch injury.

STUDY DESIGN

In vivo experiments.

METHODS

Standardised lesions were surgically induced in all four lateral suspensory branches of 12 healthy Warmblood horses. Laser therapy (class 4, 15W) was applied daily on two of four induced lesions for four consecutive weeks. Horses were randomly assigned to either short-term study (horses were sacrificed after 4 weeks) or long-term study (6 months). Suspensory ligament samples were scored after staining with haematoxylin-eosin and immunostaining for collagen 1- collagen 3- and factor VIII.

RESULTS

In the short-term study, significantly better (lower) scores for variation in density (17% above cut-off score in treated lesions vs. 31% above cut-off score in controls, P = .03), shape of nuclei (54% vs 92%, P = .02), fibre alignment (32% vs 75%, P = .003) and fibre structure (38% vs 71%, P = .02) were found in laser-treated lesions when compared to controls. Collagen 3 expression was significantly higher (32% vs 19%, P = .006) in control lesions. In both short- and long-term studies combined, parameters lesion size (44% vs 56%, P = .02) and shape of nuclei (53% vs 84%, P = .05) scored significantly better in treated lesions. Long-term, significantly better (lower) scores were found in the laser-treated group for lesion size (15% vs 45%, P = .008) and a higher percentage above cut-off score for density of the nuclei (27% vs 9%, P = .02), compared to controls.

MAIN LIMITATIONS

The model of suspensory branch injury is not an exact representation of clinical overstrain lesions.

CONCLUSIONS

These results suggest that high-power laser therapy enables better lesion healing than conservative treatment.

摘要

背景

高强度激光治疗作为一种治疗马肌腱炎和筋膜炎的再生疗法，最近受到了广泛关注。然而，缺乏评估激光治疗对大型哺乳动物组织修复的组织学影响的研究。

目的

使用悬韧带分支损伤模型，评估高强度激光治疗对悬韧带筋膜炎愈合的影响。

研究设计

体内实验。

方法

对 12 匹温血马的所有 4 个外侧悬韧带分支进行手术诱导，建立标准损伤。在 4 周的连续 4 天内，每天对 4 个诱导损伤中的 2 个进行激光治疗（4 级，15W）。马被随机分配到短期研究（4 周后处死）或长期研究（6 个月）。用苏木精-伊红染色和胶原 1-胶原 3-和因子 VIII 免疫染色对悬韧带样本进行评分。

结果

在短期研究中，与对照组相比，激光治疗组的核密度变化（治疗组的核密度变化高于截值 17%，而对照组为 31%，P=0.03）、核形状（54%比 92%，P=0.02）、纤维排列（32%比 75%，P=0.003）和纤维结构（38%比 71%，P=0.02）的评分明显更好。胶原 3 的表达在对照组中明显更高（32%比 19%，P=0.006）。在短期和长期研究的综合分析中，与对照组相比，治疗组的病变大小（44%比 56%，P=0.02）和核形状（53%比 84%，P=0.05）的参数评分显著更好。长期研究发现，与对照组相比，激光治疗组的病变大小（15%比 45%，P=0.008）和核密度高于截值的百分比（27%比 9%，P=0.02）评分显著更低。