Institute of Biosciences and Bioresources, National Research Council, 06128 Perugia, Italy.
Department of Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, Italy.
Int J Mol Sci. 2021 Dec 23;23(1):154. doi: 10.3390/ijms23010154.
Climate change, currently taking place worldwide and also in the Mediterranean area, is leading to a reduction in water availability and to groundwater salinization. Olive represents one of the most efficient tree crops to face these scenarios, thanks to its natural ability to tolerate moderate salinity and drought. In the present work, four olive cultivars (Koroneiki, Picual, Royal de Cazorla and Fadak86) were exposed to high salt stress conditions (200 mM of NaCl) in greenhouse, in order to evaluate their tolerance level and to identify key genes involved in salt stress response. Molecular and physiological parameters, as well as plant growth and leaves' ions Na and K content were measured. Results of the physiological measurements showed Royal de Cazorla as the most tolerant cultivar, and Fadak86 and Picual as the most susceptible ones. Ten candidate genes were analyzed and their complete genomic, CDS and protein sequences were identified. The expression analysis of their transcripts through reverse transcriptase quantitative PCR (RT-qPCR) demonstrated that only , , and were upregulated in tolerant cultivars, thus suggesting their key role in the activation of a salt tolerance mechanism.
气候变化正在全球范围内发生，地中海地区也受到了影响，这导致了可用水资源的减少和地下水的盐渍化。橄榄树是应对这些情况的最有效树木作物之一，这要归功于其耐受适度盐度和干旱的天然能力。在本工作中，将四个橄榄品种（科罗内利、皮库阿尔、罗亚尔德卡索拉和法达克 86）在温室中暴露于高盐胁迫条件（200 mM NaCl）下，以评估它们的耐受水平，并鉴定参与盐胁迫反应的关键基因。测量了分子和生理参数，以及植物生长和叶片中 Na 和 K 离子的含量。生理测量的结果表明，罗亚尔德卡索拉是最耐受的品种，法达克 86 和皮库阿尔是最敏感的品种。分析了 10 个候选基因，并鉴定了它们完整的基因组、CDS 和蛋白质序列。通过逆转录定量 PCR（RT-qPCR）对其转录本的表达分析表明，只有 、 、 和 在耐受品种中上调，因此表明它们在激活耐盐机制中的关键作用。
