Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi'an 710119, China.
College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China.
Int J Mol Sci. 2022 Jan 3;23(1):511. doi: 10.3390/ijms23010511.
K-homologous (KH) family is a type of nucleic acid-binding protein containing the KH domain and has been found to affect splicing and transcriptional regulation. However, KH family genes haven't been investigated in plant species systematically. In this study, we identified 30 genes that belonged to the KH family based on HMM of the KH domain in . Phylogenetic tree analysis showed that the KH family is grouped into three subgroups. Synteny analysis showed that and have the conserved synteny relationship between and the other five species. The and were located in the cytoplasm and nucleus. The seed germination rates of the mutants and were higher than wild-type after abscisic acid (ABA) and salicylic acid (SA) treatments. In addition, the expression of ABA-related genes, such as (), (), and (), and an SA-related gene () were downregulated after ABA and SA treatments, respectively. These results suggested that and mutants inhibit the effect of ABA and SA on seed germination. In conclusion, our results provide valuable information for further exploration of the function of KH family genes and propose directions and ideas for the identification and characterization of KH family genes in other plants.
KH 同源家族是一类含有 KH 结构域的核酸结合蛋白，已被发现影响剪接和转录调控。然而，KH 家族基因在植物物种中尚未得到系统研究。在这项研究中，我们基于. 的 KH 结构域的 HMM 鉴定了 30 个属于 KH 家族的基因。系统发育树分析表明，KH 家族分为三个亚组。共线性分析表明， 和 与其他五个物种之间具有保守的共线性关系。 和 位于细胞质和细胞核中。在脱落酸（ABA）和水杨酸（SA）处理后，突变体 和 的种子发芽率高于野生型。此外，ABA 相关基因如 ()、()和 ()和一个 SA 相关基因 ()的表达在 ABA 和 SA 处理后分别下调。这些结果表明 和 突变体抑制了 ABA 和 SA 对种子萌发的作用。总之，我们的研究结果为进一步探索 KH 家族基因的功能提供了有价值的信息，并为其他植物中 KH 家族基因的鉴定和特征分析提出了方向和思路。
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